Related Experiment Video
Updated: Jan 22, 2026

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
Published on: April 10, 2018
Long non-coding RNA LncKdm2b regulates cortical neuronal differentiation by cis-activating Kdm2b
Wei Li1, Wenchen Shen1, Bo Zhang1
1College of Life Sciences, Renmin Hospital of Wuhan University, Medical Research Institute at School of Medicine, Wuhan University, Wuhan, 430072, China.
This study reveals a novel long non-coding RNA, LncKdm2b, essential for regulating cortical neuron differentiation and migration. LncKdm2b controls Kdm2b expression, impacting brain development.
Area of Science:
- Neuroscience
- Molecular Biology
- Genetics
Background:
- Cortical neurogenesis mechanisms remain unclear.
- Long non-coding RNAs (lncRNAs) are key regulators of cell fate.
- Understanding lncRNA roles in brain development is crucial.
Purpose of the Study:
- To investigate the role of lncRNAs in cortical projection neuron differentiation.
- To identify novel lncRNAs involved in brain development.
- To elucidate the regulatory mechanisms of cortical neurogenesis.
Main Methods:
- Identified and characterized LncKdm2b (Kancr), a novel lncRNA.
- Investigated the regulatory relationship between LncKdm2b and Kdm2b.
- Utilized lineage tracing and phenotypic analyses.
Main Results:
- LncKdm2b is transiently expressed during early cortical neuron differentiation.
- LncKdm2b positively regulates Kdm2b transcription by creating a permissive chromatin environment.
- LncKdm2b and Kdm2b are vital for proper cortical projection neuron differentiation and migration.
Conclusions:
- A lncRNA-dependent regulatory network controls cortical neuronal differentiation.
- LncKdm2b plays a critical role in brain development by modulating neurogenesis.
- This finding opens new avenues for understanding neurological disorders.
More Related Videos
09:40Identification of Coding and Non-coding RNA Classes Expressed in Swine Whole Blood
Published on: November 28, 2018
12:44Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis
Published on: November 11, 2014
Related Concept Videos
Cis-regulatory Sequences
Regulated mRNA Transport
lncRNA - Long Non-coding RNAs
Master Transcription Regulators
Co-activators and Co-repressors
Regulation of Expression Occurs at Multiple Steps
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...