Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

From DNA to Protein

From DNA to Protein

The flow of genetic information in cells from DNA to mRNA to protein is described by the central dogma, which states that genes specify the sequence of mRNAs, which in turn specify the sequence of amino acids making up all proteins. The decoding of one molecule to another is performed by specific proteins and RNAs. Because the information stored in DNA is so central to cellular function, it makes intuitive sense that the cell would make mRNA copies of this information for protein synthesis...

Law of Segregation

Law of Segregation

When crossing pea plants, Mendel noticed that one of the parental traits would sometimes disappear in the first generation of offspring, called the F1 generation, and could reappear in the next generation (F2). He concluded that one of the traits must be dominant over the other, thereby causing masking of one trait in the F1 generation. When he crossed the F1 plants, he found that 75% of the offspring in the F2 generation had the dominant phenotype, while 25% had the recessive phenotype.

Segregation in Fresh Concrete

Segregation in Fresh Concrete

Segregation in fresh concrete is a phenomenon where the components of the concrete mix separate, leading to uneven distribution and compromised structural integrity. This separation typically occurs when concrete is subjected to excessive horizontal movement within forms, or when it is dropped from considerable heights or forced through narrow, winding paths. As a result, heavier coarse aggregate particles settle at the bottom, while lighter, finer materials such as cement and water rise to the...

Single-Strand DNA Binding Proteins

Single-Strand DNA Binding Proteins

For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...

DNA Helicases

DNA Helicases

DNA unwinding helicase enzymes are a type of motor protein. Motor proteins can translocate along filaments or polymers using energy generated from ATP hydrolysis. Helicases are involved in all the important cellular processes where DNA unwinding is required, such as DNA replication, repair, recombination, and transcription. They are present in all living organisms, but vary in their structure, function, and mechanism of action. For example, in prokaryotes, DnaB helicase binds and translocates...

DNA Packaging

DNA Packaging

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Inference of transcriptional regulation from STARR-seq data.

Physical review. E·2025

Same author

Neural basis of cognitive control signals in anterior cingulate cortex during delay discounting.

bioRxiv : the preprint server for biology·2024

Same author

Extensive androgen receptor enhancer heterogeneity in primary prostate cancers underlies transcriptional diversity and metastatic potential.

Nature communications·2022

Same author

Optimizing Efficiency and Motility of a Polyvalent Molecular Motor.

Micromachines·2022

Same author

Functional mapping of androgen receptor enhancer activity.

Genome biology·2021

Same author

Substrate stiffness tunes the dynamics of polyvalent rolling motors.

Soft matter·2020

Search research articles

Related Experiment Video

Updated: Jan 22, 2026

PAR-CliP - A Method to Identify Transcriptome-wide the Binding Sites of RNA Binding Proteins

PAR-CliP - A Method to Identify Transcriptome-wide the Binding Sites of RNA Binding Proteins

Published on: July 2, 2010

DNA segregation under Par protein control.

Lavisha Jindal¹, Eldon Emberly¹

¹Physics Department, Simon Fraser University, Burnaby, British Columbia, V5A 1S6, Canada.

|July 19, 2019

Summary

This summary is machine-generated.

Bacterial DNA segregation relies on the Par protein system. This study models ParA-ParB dynamics, revealing that oscillatory or stable DNA arrangements depend on ParB-complex number, substrate length, and ParA concentration.

More Related Videos

Monitoring Spatial Segregation in Surface Colonizing Microbial Populations

Monitoring Spatial Segregation in Surface Colonizing Microbial Populations

Published on: October 29, 2016

Live Cell Imaging of Chromosome Segregation During Mitosis

Live Cell Imaging of Chromosome Segregation During Mitosis

Published on: March 14, 2018

Related Experiment Videos

Last Updated: Jan 22, 2026

PAR-CliP - A Method to Identify Transcriptome-wide the Binding Sites of RNA Binding Proteins

PAR-CliP - A Method to Identify Transcriptome-wide the Binding Sites of RNA Binding Proteins

Published on: July 2, 2010

Monitoring Spatial Segregation in Surface Colonizing Microbial Populations

Monitoring Spatial Segregation in Surface Colonizing Microbial Populations

Published on: October 29, 2016

Live Cell Imaging of Chromosome Segregation During Mitosis

Live Cell Imaging of Chromosome Segregation During Mitosis

Published on: March 14, 2018

Area of Science:

Microbiology and Molecular Biology
Biophysics
Systems Biology

Background:

The Par protein system is crucial for bacterial DNA spatial organization and segregation.
ParB binds to specific DNA sequences (parS), interacting with ParA to move DNA.
Observed plasmid dynamics range from oscillations to stable, equidistant arrangements, but the underlying mechanisms remain unclear.

Purpose of the Study:

To investigate the stability of ParB-complex oscillations and their dependence on system parameters.
To determine how substrate length and the number of ParB complexes influence DNA segregation dynamics.
To explore the role of ParA concentration and hydrolysis in transitioning between oscillatory and fixed-point dynamics.

Main Methods:

Development of a deterministic mathematical model for ParA-ParB driven DNA segregation.
Analysis of system dynamics based on five key parameters: ParB-complex number, substrate length, ParA concentration, ParA hydrolysis rate, and lengthscale ratios.
Simulation of scenarios with buffered ParA rebinding and limited ParA resources.

Main Results:

In buffered systems, ParB-complex dynamics are largely independent of substrate length and complex number above a minimum size.
Under limited ParA resources, substrate length and complex number exert counteracting effects on oscillatory dynamics.
The transition between stable and oscillatory dynamics is governed by five critical parameters.

Conclusions:

Bacterial cells may utilize critical ParA levels to switch between oscillatory and fixed-point dynamics for cell cycle progression and DNA partitioning.
The model captures observed differences in ParB-complex positioning between bacterial chromosomes and plasmids by adjusting ParA availability or depletion zone size.
This work provides insights into the quantitative principles governing bacterial DNA segregation mechanisms.