Site-Specific Incorporation of a Dithiolane Containing Amino Acid into Proteins
Minseob Koh1, Hyeon-Yeol Cho2, Chenguang Yu1
1Department of Chemistry and Skaggs Institute for Chemical Biology , The Scripps Research Institute , 10550 N. Torrey Pines Road , La Jolla , California 92037 , United States.
Abstract:
We have genetically encoded a dithiolane containing amino acid (dtF) in Escherichia coli (E. coli) using a polyspecific aminoacyl-tRNA synthetase (aaRS)/amber suppressor tRNA pair. To demonstrate the utility of dtF for bioapplications, we synthesized gold nanoparticle (AuNP) constructs with a mutant superfolder green fluorescent protein (sfGFP) [sfGFP-AuNP] as a model for the protein-metal conjugation. The resulting sfGFP-AuNP constructs show directional homogeneity and enhanced chemical durability compared to their cysteine analogues toward excess environmental 1,4-dithiothreitol (DTT).
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