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Extraction of natural astaxanthin from Haematococcus pluvialis using liquid biphasic flotation system
Kuan Shiong Khoo1, Kit Wayne Chew1, Chien Wei Ooi2
1Department of Chemical and Environmental Engineering, Faculty of Science and Engineering, University of Nottingham Malaysia, Jalan Broga, Semenyih 43500, Selangor Darul Ehsan, Malaysia.
Abstract:
This work aimed to study the application of liquid biphasic flotation (LBF) for the efficient and rapid recovery of astaxanthin from H. pluvialis microalgae. The performance of LBF for the extraction of astaxanthin was studied comprehensively under different operating conditions, including types and concentrations of food-grade alcohol and salt, volume ratio, addition of neutral salt, flotation period, and mass of dried H. pluvialis biomass powder. The maximum recovery, extraction efficiency and partition coefficient of astaxanthin obtained from the optimum LBF system were 95.11 ± 1.35%, 99.84 ± 0.05% and 385.16 ± 3.87, respectively. A scaled-up LBF system was also performed, demonstrating the feasibility of extracting natural astaxanthin from microalgae at a larger scale. This exploration of LBF system opens a promising avenue to the extraction of astaxanthin at lower cost and shorter processing time.
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