Related Experiment Video
Updated: Jan 22, 2026

Extraction of High Molecular Weight DNA from Microbial Mats
Published on: July 7, 2011
High voltage electric discharges treatment for high molecular weight hemicelluloses extraction from spruce
Morad Chadni1, Nabil Grimi2, Isabelle Ziegler-Devin3
1Sorbonne University, Université de technologie de Compiègne, ESCOM, EA 4297 TIMR, Centre de recherche Royallieu, CS 60 319, 60 203, Compiègne cedex, France; Sofren Groupe, 1 rue Royale, 92210, Saint-Cloud, France.
Abstract:
The aim of this work is to extract hemicelluloses polymers from spruce sawdust using High Voltage Electrical Discharges (HVED) pretreatment. HVED pretreatment was carried out in order to improve deconstruction of the wood matrix and to allow easier release of high molecular mass hemicelluloses. The results revealed the advantage of HVED activation step in the process of extraction. The yield of hemicellulose extraction increased with HVED treatment time. In fact, after 4 ms of HVED treatment time in 1 M NaOH solution, the yield obtained was higher as compared to the untreated sample (19 mg/g of dry matter and 15.8 mg/g of dry matter respectively). The molecular weights of extracted hemicelluloses are very high (55 kDa and 47 kDa for 4 ms of HVED-treatment in 1 M NaOH solution and water respectively). The use of different mediums of extraction with HVED pretreatment have revealed a selective solubilization of spruce hemicelluloses.
More Related Videos
Related Concept Videos
Polymers: Defining Molecular Weight
The number average molecular weight (Mn) is the summation of the number...
Polymers: Molecular Weight Distribution
Molecular Weight of Step-Growth Polymers
As the step-growth polymerization involves step-wise condensation of monomers, the molecular weight also builds up eventually. Consequently, high molecular weight polymers are obtained at the late stages of the polymerization, where 99% of monomers have been consumed.
The extent of the...
Anticoagulant Drugs: Low-Molecular-Weight Heparins
Discharge Summary Forms
Here's a detailed look at the key components and guidelines for preparing a discharge summary:
Voltage

