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Potential transmission sources of Helicobacter pylori infection: detection of H. pylori in various environmental

Marzieh Farhadkhani1, Mahnaz Nikaeen2, Akbar Hassanzadeh3

  • 11Student Research Committee, School of Health, Isfahan University of Medical Sciences, Isfahan, Iran.

Journal of Environmental Health Science & Engineering
|July 20, 2019
PubMed
Abstract

Insights

Helicobacter pylori was detected in wastewater, suggesting it may spread infection. Sewage sludge and water samples showed lower presence, and fecal coliforms did not correlate with H. pylori.

Area of Science:

  • Environmental microbiology
  • Molecular biology
  • Public health

Background:

  • Helicobacter pylori is a common human pathogen.
  • Waterborne transmission of H. pylori is a potential public health concern.
  • Understanding environmental reservoirs is crucial for controlling H. pylori infections.

Purpose of the Study:

  • To investigate the presence of H. pylori in aquatic environments and sewage sludge using molecular methods.
  • To assess the role of these environments in H. pylori transmission.
  • To compare the sensitivity of different PCR techniques for H. pylori detection.

Main Methods:

  • Nested and semi-nested PCR targeting H. pylori 16S rRNA and ureA genes.
  • Comparison of semi-nested PCR with real-time PCR for H. pylori detection sensitivity.
  • Fecal coliform analysis as an indicator of fecal pollution.

Main Results:

  • H. pylori 16S rRNA gene detected in 36% of wastewater and 8% of water samples.
  • UreA gene amplification yielded only two positive results.
  • No H. pylori detected in sewage sludge; real-time PCR was not effective for environmental samples. No correlation found with fecal coliforms.

Conclusions:

  • Wastewater serves as a significant reservoir for H. pylori dissemination.
  • Further research is required to evaluate risks associated with wastewater reuse for crop irrigation.
  • Molecular detection methods are essential for identifying H. pylori in environmental matrices.
Keywords:
Helicobacter pyloriPCRSludgeWastewaterWater

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