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Downregulation of TCEAL7 expression induces CCND1 expression in non-small cell lung cancer
Ceren Orhan1, Pelin Bulut1, Nejat Dalay1
1Department of Medical Biology, Cerrahpasa Medical Faculty, Istanbul University-Cerrahpasa, Cerrahpasa Street, Kocamustafapasa, Fatih, 34098, Istanbul, Turkey.
Abstract:
Transcription Elongation Factor A-like 7 (TCEAL7) was first reported as a candidate tumor suppressor gene because of its inactivation in ovarian cancer as a result of promoter methylation. Down-regulation of the TCEAL7 gene expression was also associated with other cancers such as endometrial, breast, brain, prostate, gastric cancers, glioblastoma and linked to tumor phenotypes and clinical outcomes. However, there is no report in the literature investigating the role of TCEAL7 in non-small cell lung cancer. Cyclin D1 is an important molecule in the transition from G1 to S phase of the cell cycle, and is frequently deregulated in cancers. Cylin D1 (CCND1) gene is amplified or overexpressed in a variety of tumors. In our previous study we reported that CCND1 over-expression was not associated with amplification in non-small cell lung cancer. Recently, it has been reported that TCEAL7 regulates CCND1 expression through myc-binding E-box sequences. The aim of this study was to investigate the expression of TCEAL7 gene in non-small cell lung cancer and to determine its effect on the CCND1 expression level. For this purpose, expression levels of TCEAL7 and CCND1 genes were investigated in 50 patients with non-small cell lung cancer by quantitative real time polymerase chain reaction (qRT-PCR). TCEAL7 was under-expressed (68%) in non-small cell lung cancer tumor tissues while CCND1 was over-expressed (42%). The TCEAL7 levels negatively correlated with increased CCND1 expression (p = 0.002).
Insights
Transcription Elongation Factor A-like 7 (TCEAL7) is under-expressed in non-small cell lung cancer, correlating with increased Cyclin D1 (CCND1) expression. This suggests TCEAL7 may act as a tumor suppressor by regulating CCND1 in lung cancer.
Area of Science:
- Oncology
- Molecular Biology
- Gene Expression
Background:
- Transcription Elongation Factor A-like 7 (TCEAL7) is implicated as a tumor suppressor in various cancers due to promoter methylation and down-regulation.
- Cyclin D1 (CCND1), a cell cycle regulator, is frequently deregulated in tumors.
- TCEAL7 has been recently reported to regulate CCND1 expression via myc-binding E-box sequences.
Purpose of the Study:
- To investigate the expression of TCEAL7 in non-small cell lung cancer (NSCLC).
- To determine the effect of TCEAL7 expression on CCND1 levels in NSCLC.
- To explore the potential role of TCEAL7 as a tumor suppressor in NSCLC.
Main Methods:
- Quantitative real-time polymerase chain reaction (qRT-PCR) was used to analyze TCEAL7 and CCND1 gene expression.
- Gene expression levels were assessed in tumor tissues from 50 NSCLC patients.
Main Results:
- TCEAL7 was under-expressed in 68% of NSCLC tumor tissues.
- CCND1 was over-expressed in 42% of NSCLC tumor tissues.
- A significant negative correlation was observed between TCEAL7 levels and CCND1 expression (p=0.002).
Conclusions:
- TCEAL7 is frequently under-expressed in non-small cell lung cancer.
- The down-regulation of TCEAL7 is associated with increased CCND1 expression in NSCLC.
- These findings suggest TCEAL7 may function as a tumor suppressor in NSCLC by modulating CCND1 expression.
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