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Long non-coding RNA LINC00641 suppresses non-small-cell lung cancer by sponging miR-424-5p to upregulate PLSCR4

Yi Li, Lily Zhao, Pei Zhao

    Cancer Biomarkers : Section a of Disease Markers
    |July 20, 2019
    PubMed

    Insights

    Long non-coding RNA LINC00641 acts as a tumor suppressor in non-small cell lung cancer (NSCLC). It inhibits NSCLC cell proliferation and promotes apoptosis by sponging miR-424-5p to regulate PLSCR4 expression.

    Area of Science:

    • Oncology
    • Molecular Biology
    • Genetics

    Background:

    • Non-small cell lung cancer (NSCLC) is a prevalent and severe malignancy requiring new therapies.
    • Long non-coding RNAs (lncRNAs) are implicated in cancer, but the role of LINC00641 in NSCLC is unknown.
    • Previous studies suggest LINC00641 inhibits bladder cancer progression.

    Purpose of the Study:

    • To investigate the function and molecular mechanisms of LINC00641 in NSCLC.
    • To determine if LINC00641 acts as a tumor suppressor or oncogene in NSCLC.
    • To elucidate the potential ceRNA network involving LINC00641 in NSCLC.

    Main Methods:

    • Quantitative real-time PCR to assess LINC00641 expression in NSCLC cells.
    • In vitro assays to evaluate the effects of LINC00641 on cell proliferation and apoptosis.
    • Mechanism studies involving RNA immunoprecipitation and luciferase reporter assays to confirm the ceRNA network.

    Main Results:

    • LINC00641 was found to be downregulated in NSCLC cells.
    • Overexpression of LINC00641 suppressed NSCLC cell proliferation and induced apoptosis.
    • LINC00641 functions as a competing endogenous RNA (ceRNA) by sponging miR-424-5p, leading to the upregulation of PLSCR4.

    Conclusions:

    • LINC00641 acts as a tumor suppressor in NSCLC.
    • The LINC00641/miR-424-5p/PLSCR4 axis plays a critical role in NSCLC progression.
    • LINC00641 may represent a potential therapeutic target for NSCLC treatment.
    Keywords:
    LINC00641NSCLCPLSCR4miR-424-5p

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