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Preparation of Exosomes for siRNA Delivery to Cancer Cells
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Nanotechnology-based siRNA delivery strategies for metastatic colorectal cancer therapy

Ana Rita Sousa1, Ana Vanessa Oliveira2, Maria José Oliveira3

  • 1i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Rua Alfredo Allen, 208, 4200-135 Porto, Portugal; INEB - Instituto de Engenharia Biomédica, Universidade do Porto, Rua Alfredo Allen, 208, 4200-135 Porto, Portugal; IPO Porto - Instituto Português de Oncologia do Porto, Rua Dr António Bernardino de Almeida, 4200-072 Porto, Portugal; ICBAS - Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, Rua Jorge de Viterbo Ferreira, 228, 4050-313 Porto, Portugal.

International Journal of Pharmaceutics
|July 20, 2019
PubMed

Insights

Developing effective treatments for metastatic colorectal cancer (mCRC) is crucial. This study explores using poly(lactic-co-glycolic acid) nanoparticles to deliver small interfering RNA (siRNA) for targeted mCRC therapy.

Area of Science:

  • Oncology
  • Nanotechnology
  • Molecular Biology

Background:

  • Colorectal cancer (CRC) is a leading cause of cancer death, primarily due to metastasis.
  • Metastatic CRC (mCRC) presents significant therapeutic resistance.
  • Novel strategies are essential to combat advanced CRC.

Purpose of the Study:

  • To review strategies for overcoming barriers in systemic small interfering RNA (siRNA) delivery for mCRC.
  • To highlight the potential of poly(lactic-co-glycolic acid) (PLGA) nanoparticles for siRNA delivery in mCRC.
  • To summarize methods for encapsulating siRNA into PLGA nanocarriers.

Main Methods:

  • Discussion of extracellular and intracellular barriers to intravenous siRNA delivery.
  • Emphasis on combining siRNA with nanoparticles for sustained release and reduced toxicity.
  • Review of strategies for siRNA encapsulation within PLGA nanoparticles.

Main Results:

  • Nanoparticle-based delivery systems can enhance siRNA efficacy.
  • PLGA nanoparticles offer a promising platform for targeted siRNA delivery in mCRC.
  • Various techniques exist to overcome challenges in siRNA loading into PLGA nanocarriers.

Conclusions:

  • Systemic siRNA delivery via PLGA nanoparticles holds significant potential for mCRC treatment.
  • Further development of encapsulation strategies is needed to optimize PLGA-based siRNA delivery.
  • This approach could lead to more effective therapies for advanced colorectal cancer.
Keywords:
Co-encapsulantsGene silencingMetastatic colorectal cancerPLGA nanoparticlesRNA interference technology

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