Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Leonardo's Donna Nuda unveiled.

Elettra Barberis1, Marcello Manfredi2, Emilio Marengo1

  • 1Dipartimento di Scienze e Innovazione Tecnologica, Università del Piemonte Orientale, Novara, Italy; Center for Translational Research on Autoimmune and Allergic Diseases, Università del Piemonte Orientale, Novara, Italy.

Journal of Proteomics
|July 20, 2019
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Osmotic Remodeling of Extracellular Vesicles for Precision Nanomedicine.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

Molecular characterisation of extracellular vesicles released by Strongyloides stercoralis infective larvae isolated from a clinical sample.

Parasites & vectors·2026
Same author

Physiological and proteomic analysis reveal high recovery capacity of quinoa (Chenopodium quinoa Willd.) after progressive drought stress.

Scientific reports·2026
Same author

RAGE Re-Expressed at Myofibre Level Drives Muscle Wasting in Cancer Conditions.

Journal of cachexia, sarcopenia and muscle·2026
Same author

Vitamin D binding protein induces skeletal muscle atrophy and contributes to cancer-associated muscle wasting independently of vitamin D status in preclinical models.

Nature communications·2026
Same author

Biological Evaluation of a Novel Compound with Predicted EZH2 and EED Binding Against Human Malignant Melanoma Cells.

International journal of molecular sciences·2026
This summary is machine-generated.

Researchers used advanced EVA film technology to analyze Leonardo da Vinci's "Donna Nuda." This non-invasive method revealed his painting techniques, including tempera grassa and conifer resin protection, offering new insights into Renaissance art.

Area of Science:

  • Art Conservation Science
  • Materials Science in Art
  • Analytical Chemistry for Cultural Heritage

Background:

  • Leonardo da Vinci's "Donna Nuda," housed at the Hermitage Museum, is a significant Renaissance artwork.
  • Previous studies identified some materials used by Leonardo but lacked comprehensive analysis of his techniques.
  • Understanding Leonardo's artistic recipes is crucial for art history and conservation.

Purpose of the Study:

  • To explore the surface of "Donna Nuda" using novel analytical techniques.
  • To decipher the specific drawing and painting techniques employed by Leonardo da Vinci.
  • To investigate the materials and methods used for surface protection and glazing.

Main Methods:

  • Utilized ethylene vinyl acetate (EVA) film technology for non-invasive surface sampling.
Keywords:
Conifer resinEVA filmLeonardo da VinciTempera grassa

Related Experiment Videos

  • Applied EVA films (embedded with ion exchangers and C8/C18 resins) for 60 minutes on different painting areas.
  • Analyzed eluted materials using gas-chromatography/mass spectrometry (GC/MS) and liquid chromatography/mass spectrometry (LC/MS).
  • Main Results:

    • Confirmed the use of "tempera grassa" (linseed oil and egg yolk) throughout the painting.
    • Identified a protective layer of conifer resin on the painting's surface.
    • Detected rosemary oil as a diluent, used to facilitate glazing and achieve aerial perspective in the landscape.

    Conclusions:

    • This study provides the first in-depth analysis of a Leonardo painting using modern techniques to decipher his artistic methods.
    • EVA film technology offers a powerful, non-invasive approach for analyzing cultural heritage objects.
    • The methodology has potential applications in authenticating artworks and detecting art fraud.