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Updated: Jan 22, 2026

Extraction and Analysis of Microbial Phospholipid Fatty Acids in Soils
Published on: August 26, 2016
Extraction of Dairy Phospholipids Using Switchable Solvents: A Feasibility Study
Shouyun Cheng1, Kaavya Rathnakumar1, Sergio I Martínez-Monteagudo2
1Dairy and Food Science Department, South Dakota State University, Alfred Dairy Science Hall, Brookings, SD 57007, USA.
Switchable hydrophilicity solvents (SHS) efficiently extract phospholipids (PLs) from dairy byproducts. This novel method significantly outperforms traditional techniques, offering a promising approach for PL recovery.
Area of Science:
- Chemistry
- Food Science
- Biotechnology
Background:
- Phospholipids (PLs) are valuable compounds found in dairy byproducts.
- Traditional extraction methods for PLs can be inefficient and environmentally taxing.
Purpose of the Study:
- To evaluate the efficacy of a switchable hydrophilicity solvent (SHS) for phospholipid extraction from dairy byproducts.
- To compare the SHS method with conventional Folch (FE) and Mojonnier (ME) extraction techniques.
Main Methods:
- Utilized N,N-dimethylcyclohexylamine (CyNMe2) as an SHS for phospholipid extraction.
- Performed extractions on raw cream, buttermilk, concentrated buttermilk, and beta-serum.
- Recovered extracted phospholipids by switching the solvent's hydrophilicity with CO2.
Main Results:
- SHS extraction efficiency varied from 0.33% to 99% across different dairy byproducts.
- Achieved up to 99% PL extraction from buttermilk using SHS.
- Demonstrated significantly higher PL recovery compared to FE and ME methods.
Conclusions:
- Switchable hydrophilicity solvents offer a highly effective and applicable method for phospholipid extraction from dairy byproducts.
- The SHS approach presents a superior alternative to conventional methods for PL recovery.
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