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Simultaneous quantification of six indicator compounds in Wen-Qing-Yin by high-performance liquid
Chi-Chen Yeh1, Shih-Shan Huang1, Po-Yu Liu2,3
1Division of Research and Analysis, Food and Drug Administration, Ministry of Health and Welfare, Executive Yuan, 161-2 Kunyang St., Nangang Dist., Taipei City 11561, Taiwan.
Journal of Food and Drug Analysis
|July 21, 2019
Summary
A new HPLC-DAD method accurately analyzes six key compounds in Wen-Qing-Yin (WQY), a traditional Chinese medicine. This validated approach ensures reliable quality control for WQY decoctions and commercial products.
Area of Science:
- Analytical Chemistry
- Pharmacognosy
- Traditional Chinese Medicine (TCM) Analysis
Background:
- Traditional Chinese Medicine (TCM) formulations like Wen-Qing-Yin (WQY) require robust analytical methods for quality control.
- Simultaneous analysis of multiple active compounds in complex TCM matrices presents significant challenges.
- Establishing validated methods is crucial for ensuring the safety and efficacy of TCM products.
Purpose of the Study:
- To develop and validate a simple gradient high-performance liquid chromatography with diode array detection (HPLC-DAD) method.
- To enable simultaneous qualitative and quantitative analysis of six indicator compounds in Wen-Qing-Yin (WQY).
- To assess the method's suitability for analyzing commercial WQY decoctions.
Main Methods:
- A gradient HPLC-DAD method was optimized using a Cosmosil C18 column with 0.1% formic acid in aqueous and acetonitrile mobile phases.
- Method parameters included a flow rate of 1.8 mL/min, detection at 268 nm, and a sample volume of 10 μL.
- Validation encompassed linearity, precision, recovery, and sensitivity (LOD/LOQ) for berberine, baicalin, ferulic acid, geniposide, hydroxymethoxylfurfural, and paeoniflorin.
Main Results:
- The method demonstrated excellent linearity (R² from 0.9966 to 0.9989) across a concentration range of 10-2000 μg/mL.
- Validation showed acceptable precision (<4.11%) and recovery (86.61-109.42%).
- Sensitivity was adequate, with LOD and LOQ values in the μg/mL range. Principal Component Analysis (PCA) successfully differentiated commercial WQY samples.
Conclusions:
- The developed HPLC-DAD method is simple, efficient, and suitable for the simultaneous qualitative and quantitative analysis of six indicator compounds in WQY.
- The method provides reliable results for assessing the quality of WQY decoctions and their commercial products.
- The validated method supports the standardization and quality control of this important TCM formulation.
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