Related Experiment Video

Updated: Jan 22, 2026

Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells
16:24

Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells

Published on: February 21, 2014

20.7K

Delphinidin suppresses breast carcinogenesis through the HOTAIR/microRNA-34a axis

Bin Han1, Xiaoli Peng1, Daomei Cheng1

  • 1School of Public Health, Chengdu Medical College, Chengdu, China.

Cancer Science
|July 21, 2019
PubMed

Insights

Delphinidin, a plant compound, effectively suppresses breast cancer in rats by regulating the HOTAIR/microRNA-34a pathway. This natural compound shows promise for preventing breast carcinogenesis.

Area of Science:

  • Oncology
  • Molecular Biology
  • Natural Products Chemistry

Background:

  • Anthocyanidins, like delphinidin, exhibit significant anti-cancer properties.
  • Breast cancer remains a leading cause of mortality, necessitating novel therapeutic strategies.
  • Understanding molecular mechanisms underlying cancer development is crucial for prevention and treatment.

Purpose of the Study:

  • To investigate the anti-cancer effects of delphinidin on 1-methyl-1-nitrosourea (MNU)-induced breast carcinogenesis in rats.
  • To elucidate the molecular mechanism involving the HOTAIR/microRNA-34a axis in delphinidin's action.
  • To evaluate delphinidin's impact on key signaling pathways implicated in breast cancer progression.

Main Methods:

  • MNU-induced rat breast cancer model.
  • Delphinidin administration and assessment of tumor suppression.
  • Analysis of HOTAIR and microRNA-34a expression levels.
  • Western blot analysis for Wnt/β-catenin pathway proteins (β-catenin, Gsk3β, c-Myc, cyclin-D1) and MMP-7.
  • RNA immunoprecipitation (RIP) and chromatin immunoprecipitation (ChIP) assays.

Main Results:

  • Delphinidin significantly suppressed MNU-induced breast carcinogenesis in rats.
  • Delphinidin downregulated HOTAIR and upregulated microRNA-34a expression.
  • Delphinidin reduced the expression of β-catenin, Gsk3β, c-Myc, cyclin-D1, and MMP-7.
  • MicroRNA-34a inhibition diminished delphinidin's effects on c-Myc, cyclin-D1, and MMP-7.
  • HOTAIR overexpression counteracted delphinidin's effects on microRNA-34a and the Wnt/β-catenin pathway.
  • Delphinidin enhanced EZH2 and H3K27me3, indicating epigenetic regulation of HOTAIR/microRNA-34a.

Conclusions:

  • Delphinidin demonstrates potent anti-cancer activity against breast carcinogenesis.
  • The anti-cancer mechanism involves negative regulation of the HOTAIR/microRNA-34a axis.
  • Delphinidin's action is linked to the modulation of the Wnt/β-catenin signaling pathway.
  • These findings support delphinidin's potential as a preventive agent for breast cancer.
Keywords:
HOTAIRWnt/β-catenin signaling pathwaybreast carcinogenesisdelphinidinmicroRNA-34a

More Related Videos

Loss-of-Function Approach in the Embryonic Chick Retina by Using Tol2 Transposon-Mediated Transgenic Expression of Artificial microRNAs06:58

Loss-of-Function Approach in the Embryonic Chick Retina by Using Tol2 Transposon-Mediated Transgenic Expression of Artificial microRNAs

Published on: May 18, 2022

1.4K
Orthotopic Transplantation of Breast Tumors as Preclinical Models for Breast Cancer07:45

Orthotopic Transplantation of Breast Tumors as Preclinical Models for Breast Cancer

Published on: May 18, 2020

6.9K

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
24.0K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.8K
Hypothalamic-Pituitary Axis01:37

Hypothalamic-Pituitary Axis

The response to stress—be it physical or psychological, acute or chronic—involves activation of the Hypothalamic-Pituitary-Adrenal (HPA) axis. The HPA axis is part of the neuroendocrine system because it involves both neuronal and hormonal communication. Its function is to regulate homeostatic systems—metabolic, cardiovascular, and immune—providing the necessary means to respond to a stressor.
65.6K
Perpendicular-Axis Theorem01:16

Perpendicular-Axis Theorem

The perpendicular-axis theorem states that the moment of inertia of a planar object about an axis perpendicular to its plane is equal to the sum of the moments of inertia about two mutually perpendicular concurrent axes lying in the plane of the body.
Consider a circular disc of mass M and radius R lying along an x-y plane. The origin lies at the center of the disc, and the z-axis is perpendicular to the disc's plane. All three axes coincide at the disc's center. The moment of inertia of this...
4.5K
Parallel-axis Theorem01:06

Parallel-axis Theorem

The parallel-axis theorem provides a convenient and quick method of finding the moment of inertia of an object about an axis parallel to the axis passing through its center of mass. Consider a thin rod as an example. There is a striking similarity between the process of finding the moment of inertia of a thin rod about an axis through its middle, where the center of mass lies, and about an axis through its end using the conventional method. In the conventional method, the concept of linear mass...
8.2K
Moment of Inertia about an Arbitrary Axis01:20

Moment of Inertia about an Arbitrary Axis

The moment of inertia is typically associated with principal axes, but it can also be computed for any random axis. When an arbitrary axis is under consideration, the moment of inertia is determined by integrating the mass distribution of the object along that specific axis. It is crucial in applications like the design of machinery, where components rotate about various axes, and balance and stability are essential.
In this scenario, the perpendicular distance between the chosen arbitrary axis...
617