Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mesenchymal Stem Cells01:19

Mesenchymal Stem Cells

5.5K
Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...
5.5K
Globular and Fibrous Proteins02:21

Globular and Fibrous Proteins

46.8K
Many proteins can be classified into two distinct subtypes - globular or fibrous. These two types differ in their shapes and solubilities.
Globular proteins are also known as spheroproteins and typically are approximately round in shape. They contain a mix of amino acid types and contain differing sequences in their primary structures. Globular proteins have many different functions, such as enzymes, cellular messengers, and molecular transporters. These roles often require the proteins to be...
46.8K
Adult Stem Cells01:33

Adult Stem Cells

33.4K
Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously...
33.4K
Embryonic Stem Cells00:58

Embryonic Stem Cells

32.1K
Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
32.1K
Embryonic Stem Cells00:57

Embryonic Stem Cells

4.7K
Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
4.7K
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

27.3K
Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
27.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Material-Induced Nuclear Deformation Controls Chromatin Architecture in Adipose Stem Cells.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Leveraging agent-based models and deep reinforcement learning to predict taxis in cell migration.

NPJ systems biology and applications·2025
Same author

Flow-induced mechano-modulation of intestinal permeability on chip.

Materials today. Bio·2025
Same author

Regulation of nuclear deformation via spatiotemporal modulation of cytoskeleton forces on photo-switchable surfaces.

Biomaterials·2025
Same author

A Half-Sandwich Os(II) Glucoconjugated NHC Complex as a Modulator of Amyloid Aggregation.

Inorganic chemistry·2025
Same author

Understanding and measuring mechanical signals in the tumor stroma.

FEBS open bio·2024

Related Experiment Video

Updated: Jan 22, 2026

Derivation and Differentiation of Canine Ovarian Mesenchymal Stem Cells
11:41

Derivation and Differentiation of Canine Ovarian Mesenchymal Stem Cells

Published on: December 16, 2018

6.0K

Aligned fibrous decellularized cell derived matrices for mesenchymal stem cell amplification.

Maurizio Ventre1,2,3, Valerio Coppola1, Carlo F Natale2

  • 1Department of Chemical, Materials and Industrial Production Engineering, University of Naples Federico II, Naples, Italy.

Journal of Biomedical Materials Research. Part A
|July 21, 2019
PubMed
Summary

Cell-derived matrices guide stem cell fate. Matrix stiffness and alignment control stem cell self-renewal and differentiation, offering new methods for stem cell research and applications.

Keywords:
aligned fiberscell derived matricesdecellularizationmesenchymal stem cells

More Related Videos

Preparation of 3D Decellularized Matrices from Fetal Mouse Skeletal Muscle for Cell Culture
07:44

Preparation of 3D Decellularized Matrices from Fetal Mouse Skeletal Muscle for Cell Culture

Published on: March 3, 2023

1.8K
Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells
22:06

Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells

Published on: February 25, 2007

14.0K

Related Experiment Videos

Last Updated: Jan 22, 2026

Derivation and Differentiation of Canine Ovarian Mesenchymal Stem Cells
11:41

Derivation and Differentiation of Canine Ovarian Mesenchymal Stem Cells

Published on: December 16, 2018

6.0K
Preparation of 3D Decellularized Matrices from Fetal Mouse Skeletal Muscle for Cell Culture
07:44

Preparation of 3D Decellularized Matrices from Fetal Mouse Skeletal Muscle for Cell Culture

Published on: March 3, 2023

1.8K
Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells
22:06

Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells

Published on: February 25, 2007

14.0K

Area of Science:

  • Biomaterials Science
  • Stem Cell Biology
  • Mechanobiology

Background:

  • Stem cell niches regulate self-renewal and differentiation through biochemical and biophysical cues.
  • Replicating niche environments in vitro is challenging, limiting control over stem cell functions.
  • Cell-derived matrices offer a biomimetic approach to studying stem cell fate decisions.

Purpose of the Study:

  • To investigate the role of matrix orientation and stiffness in maintaining stem cell characteristics.
  • To analyze the impact of these factors on the adipogenic and osteogenic differentiation of murine mesenchymal stem cells (mMSCs).
  • To explore the potential of cell-derived matrices for controlled stem cell applications.

Main Methods:

  • MC3T3 cells were used to synthesize aligned fibrous matrices.
  • Decellularized matrices were employed to study mMSC behavior.
  • Matrix stiffness and fibrillar alignment were systematically varied.
  • mMSC proliferation, stemness maintenance, and differentiation potential were assessed.

Main Results:

  • Decellularized matrices enhanced mMSC proliferation.
  • Matrix stiffness and alignment synergistically influenced cell fate.
  • Soft matrices promoted stemness, while stiff matrices induced differentiation with biochemical cues.
  • Matrix alignment improved population homogeneity, reducing spontaneous adipogenesis and cross-differentiation.

Conclusions:

  • Mechanical signaling, particularly matrix stiffness and alignment, is a dominant factor in mMSC fate determination.
  • Aligned matrices create a more homogeneous environment, leading to uniform cellular responses.
  • In vitro produced cell-derived matrices provide a facile and consistent platform for stem cell amplification and mechanotransduction studies.