Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Genetic Screens02:46

Genetic Screens

5.6K
Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which...
5.6K
Cancers Originate from Somatic Mutations in a Single Cell02:21

Cancers Originate from Somatic Mutations in a Single Cell

14.6K
Cancer arises from mutations in the critical genes that allow healthy cells to escape cell cycle regulation and acquire the ability to proliferate indefinitely. Though originating from a single mutation event in one of the originator cells, cancer progresses when the mutant cell lines continue to gain more and more mutations, and finally, become malignant. For example, chronic myelogenous leukemia (CML) develops initially as a non-lethal increase in white blood cells, which progressively...
14.6K
Properties of Enantiomers and Optical Activity02:24

Properties of Enantiomers and Optical Activity

21.5K
It is essential to understand the difference between chiral and achiral interactions and the implications thereof in optical activity and their applications. Just as our feet, which are chiral, interact uniquely with chiral objects, such as a pair of shoes, but identically with achiral socks, enantiomers of a molecule exhibit different properties only when they interact with other chiral media. An example of a significant implication from this facet is the phenomenon known as optical activity,...
21.5K
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

8.9K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
8.9K
Load along a Single Axis01:29

Load along a Single Axis

632
In structural engineering, the analysis of beams subjected to varying loads is a critical aspect of understanding the behavior and performance of these structural elements. A common scenario involves a beam subjected to a combination of different load distributions.
Consider a beam of length L subjected to a varying load, which is a combination of parabolic and trapezoidal load distribution along the x-axis. In this case, it is essential to determine the resultant loads, their locations, and...
632
Single Pipe Systems01:24

Single Pipe Systems

438
In pipe flow analysis, problems are typically categorized into three types — Type I, Type II, and Type III — based on the known parameters and the desired outcome. Each type of problem addresses specific engineering requirements using fluid properties, pipe characteristics, and operational conditions.
In a Type I problem, fluid properties (density and viscosity), pipe characteristics (including diameter, length, and surface roughness), and the flow rate or average velocity are...
438

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Aerobic Exercise Mitigates Experimental Knee Osteoarthritis via Inhibition of cGAS-STING Signaling.

Microbiology and immunology·2026
Same author

Successful Preparation of Co-BTE Metal-Organic Frameworks for All-Optical Nonlinear Switching and Photonic Diode Functions.

ACS applied materials & interfaces·2026
Same author

Repeated Annual Influenza Vaccination in Older Adults Induces Comparable Seroprotection Despite Reduced Antibody Fold Rise: A 6-Month Prospective Cohort Study in China.

Vaccines·2026
Same author

Scalable generalized meta-spanners enabling parallel multitasking optical manipulation.

Science advances·2026
Same author

Metasurface Vision Transformer: A Generic AI Model for Metasurface Inverse Design.

Nanophotonics (Berlin, Germany)·2026
Same author

Comments on "Surgical Decision Making in Incisional Blepharoplasty in Asians".

Aesthetic plastic surgery·2026
Same journal

Defining Safe Light Intensity Limits of Near-Infrared Illumination Avoiding Skin Heating in Medical Optical Diagnostic Methods.

Journal of biophotonics·2026
Same journal

Review of the SWIR Windows to Study Osteoarthritis.

Journal of biophotonics·2026
Same journal

FTIR-ATR Spectroscopy as a Tool to Differentiate Listeria monocytogenes by Geno-Serogroups, Growth Conditions and Persistence Status.

Journal of biophotonics·2026
Same journal

Utilizing Serum Fluorescence Spectra and Machine Learning Algorithms for Efficient Diagnosis of Sheep Brucellosis.

Journal of biophotonics·2026
Same journal

Fluorescence Profiling of Water-Based Breast Tissue Homogenates Combined With Chemometric Analyses for Discrimination of Benign and Malignant Lesions.

Journal of biophotonics·2026
Same journal

Using Principal Components Analysis to Visualize Motion and Mitigate Artifacts in Dynamic Optical Coherence Tomography.

Journal of biophotonics·2026
See all related articles

Related Experiment Video

Updated: Jan 22, 2026

Ordering Single Cells and Single Embryos in 3D Confinement: A New Device for High Content Screening
14:22

Ordering Single Cells and Single Embryos in 3D Confinement: A New Device for High Content Screening

Published on: September 18, 2016

9.0K

Optical fan for single-cell screening.

Xiaoshuai Liu1, Yuchao Li1, Xiaohao Xu1

  • 1Institute of Nanophotonics, Jinan University, Guangzhou, China.

Journal of Biophotonics
|July 21, 2019
PubMed
Summary
This summary is machine-generated.

This study introduces an "optical fan" method for single-cell screening, effectively separating K562 leukemia cells from red blood cells using a fiber probe and laser. This technique offers an efficient approach for early disease diagnosis and treatment monitoring.

Keywords:
biophotonicscell screeningfiber probeleukemic celloptical fan

More Related Videos

Single-Cell Optical Action Potential Measurement in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
08:39

Single-Cell Optical Action Potential Measurement in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

Published on: December 22, 2020

4.7K
Fabrication of an Optical Cell Dryer for the Spectroscopic Analysis Cells
05:37

Fabrication of an Optical Cell Dryer for the Spectroscopic Analysis Cells

Published on: January 8, 2019

5.5K

Related Experiment Videos

Last Updated: Jan 22, 2026

Ordering Single Cells and Single Embryos in 3D Confinement: A New Device for High Content Screening
14:22

Ordering Single Cells and Single Embryos in 3D Confinement: A New Device for High Content Screening

Published on: September 18, 2016

9.0K
Single-Cell Optical Action Potential Measurement in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
08:39

Single-Cell Optical Action Potential Measurement in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

Published on: December 22, 2020

4.7K
Fabrication of an Optical Cell Dryer for the Spectroscopic Analysis Cells
05:37

Fabrication of an Optical Cell Dryer for the Spectroscopic Analysis Cells

Published on: January 8, 2019

5.5K

Area of Science:

  • Biomedical Engineering
  • Optical Physics
  • Cell Biology

Background:

  • Single-cell screening is crucial for early disease diagnosis and treatment monitoring in advanced biomedicine.
  • Current methods for isolating specific cell types from complex biological samples can be challenging and inefficient.
  • K562 cells (a leukemia cell line) and red blood cells (RBCs) represent a common cell population relevant to hematological studies.

Purpose of the Study:

  • To develop and demonstrate a novel "optical fan" strategy for efficient single-cell screening.
  • To isolate K562 cells from a mixture of red blood cells at the single-cell level.
  • To validate the effectiveness of the optical fan method through experimental results and numerical simulation.

Main Methods:

  • Utilized a specifically designed fiber probe with a flat facet for optical manipulation.
  • Employed a 980-nm laser beam to generate an "optical fan" effect.
  • Developed a method to selectively repel red blood cells while retaining target K562 cells.

Main Results:

  • Successfully screened K562 cells at the single-cell level from a population of red blood cells.
  • Demonstrated the capability to screen multiple leukemic cells from hundreds of red blood cells.
  • Experimental findings were corroborated by numerical simulations.

Conclusions:

  • The "optical fan" strategy provides an efficient and effective approach for single-cell screening.
  • This method holds potential for applications in early disease diagnosis and treatment monitoring.
  • Further investigation into the optical fan's stiffness parameters can optimize its performance.