Interferon-gamma and Ia antigen are present on astrocytes in active chronic multiple sclerosis lesions

Using immunocytochemical techniques, presence of interferon (IFN)-gamma, IFN-alpha and Class II major histocompatibility (MHC) antigen (HLA-DR, Ia) was evaluated in active central nervous system (CNS) lesions of 11 patients with chronic multiple sclerosis (MS) and the findings were correlated with a detailed analysis of inflammatory cells. Both IFN-gamma and Ia antigen were detectable on astrocytes at the edge of active chronic MS lesions and in the adjacent normal-appearing white matter, where CD4+ (helper/inducer) T cells and interleukin-2 receptor (IL-2R)-positive cells were also found. IFN-alpha was predominantly seen on Ia-positive macrophages and not on astrocytes. Astroglia in the inactive lesion center and in normal CNS parenchyma were unreactive for both types of IFN and Ia antigen. These findings suggested that similar to the situation in vitro, IFN-gamma can also induce Ia-expression on astrocytes in vivo. Via local antigen presentation, IFN-gamma might thus play an important role in the development of MS lesions, while IFN-alpha might be involved in local immunosuppression.