Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Interference and Diffraction02:18

Interference and Diffraction

51.8K
Interference is a characteristic phenomenon exhibited by waves. When two electromagnetic waves interact with their peaks and troughs coinciding, a resulting wave with enhanced amplitude is produced. This is known as constructive interference. In this case, the two waves interacting are in phase with each other.
51.8K
RNA Interference01:23

RNA Interference

27.8K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
27.8K
Bioavailability Study Design: Single Versus Multiple Dose Studies01:11

Bioavailability Study Design: Single Versus Multiple Dose Studies

218
Bioavailability studies are essential for understanding how a drug is absorbed, distributed, metabolized, and excreted in the body. These studies assess the extent and rate at which the active pharmaceutical agent becomes available at the site of action. The design of bioavailability studies can involve single-dose or multiple-dose regimens, each with distinct advantages and limitations.Single-dose studies are the preferred approach due to their simplicity and reduced drug exposure for...
218
Interference and Decay01:16

Interference and Decay

431
Forgetting is a complex cognitive phenomenon influenced by several factors, among which interference and decay are particularly prominent. These processes explain why individuals often struggle to retrieve specific information from memory, leading to lapses in recall that can be observed in everyday situations.
Interference occurs when competing memories hinder the retrieval of particular information. It can be classified into two types: proactive and retroactive interference. Proactive...
431
Multiple Allele Traits01:49

Multiple Allele Traits

38.0K
The Concept of Multiple Allelism
38.0K
Interference: Path Lengths01:10

Interference: Path Lengths

1.9K
Consider two sources of sound, that may or may not be in phase, emitting waves at a single frequency, and consider the frequencies to be the same.
Two special sources may be considered when they are in phase. This can be easily achieved by feeding the two sources from the same source. An example would be synchronizing the two speakers by feeding them with the same source, such as the sound waves produced by a tuning fork. This setup ensures that the two sources have the same frequency and are...
1.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Glucocorticoid Reduction After Starting Crinecerfont in Pediatric Patients With Classic CAH: Practical Perspectives.

The Journal of clinical endocrinology and metabolism·2026
Same author

Four Consecutive False Negative Newborn Screens in a Patient with Classical Congenital Adrenal Hyperplasia: A Case Report

Journal of clinical research in pediatric endocrinology·2026
Same author

Development and validation of a dual-perspective competency scale for novice medical laboratory scientists in Japan.

BMC medical education·2026
Same author

Few-shot segmentation and fiber tractography of human vagus nerve using 3D-MUSE imaging.

Proceedings of SPIE--the International Society for Optical Engineering·2025
Same author

Ophthalmic Segmentation and Analysis Software (OASIS): A Comprehensive Tool for Quantitative Evaluation of Meibography Images.

Translational vision science & technology·2025
Same author

Arterial, Venous, and Cerebrospinal Fluid Flow and Pulsatility in Stroke-Related Cerebral Small Vessel Disease: A Longitudinal Analysis.

Stroke·2025

Related Experiment Video

Updated: Jan 21, 2026

Studying Protein Function and the Role of Altered Protein Expression by Antibody Interference and Three-dimensional Reconstructions
11:57

Studying Protein Function and the Role of Altered Protein Expression by Antibody Interference and Three-dimensional Reconstructions

Published on: April 21, 2016

7.0K

Heterophile antibody interference affecting multiple Roche immunoassays: A case study.

Virian D Serei1, Ian Marshall1, Mary O Carayannopoulos1

  • 1Rutgers Robert Wood Johnson Medical School, New Brunswick, NJ, United States of America.

Clinica Chimica Acta; International Journal of Clinical Chemistry
|July 21, 2019
PubMed
Summary
This summary is machine-generated.

Heterophile antibody interference can cause inaccurate immunoassay results, leading to misdiagnosis. This case highlights the importance of investigating discordant laboratory findings with clinical presentation, especially in thyroid hormone testing.

Keywords:
Biotin-streptavidinHeterophile antibodyHyperthyroidImmunoassay interference

More Related Videos

Developing a Salivary Antibody Multiplex Immunoassay to Measure Human Exposure to Environmental Pathogens
09:08

Developing a Salivary Antibody Multiplex Immunoassay to Measure Human Exposure to Environmental Pathogens

Published on: September 12, 2016

9.1K
Immunohistochemistry and Multiple Labeling with Antibodies from the Same Host Species to Study Adult Hippocampal Neurogenesis
09:24

Immunohistochemistry and Multiple Labeling with Antibodies from the Same Host Species to Study Adult Hippocampal Neurogenesis

Published on: April 22, 2015

27.2K

Related Experiment Videos

Last Updated: Jan 21, 2026

Studying Protein Function and the Role of Altered Protein Expression by Antibody Interference and Three-dimensional Reconstructions
11:57

Studying Protein Function and the Role of Altered Protein Expression by Antibody Interference and Three-dimensional Reconstructions

Published on: April 21, 2016

7.0K
Developing a Salivary Antibody Multiplex Immunoassay to Measure Human Exposure to Environmental Pathogens
09:08

Developing a Salivary Antibody Multiplex Immunoassay to Measure Human Exposure to Environmental Pathogens

Published on: September 12, 2016

9.1K
Immunohistochemistry and Multiple Labeling with Antibodies from the Same Host Species to Study Adult Hippocampal Neurogenesis
09:24

Immunohistochemistry and Multiple Labeling with Antibodies from the Same Host Species to Study Adult Hippocampal Neurogenesis

Published on: April 22, 2015

27.2K

Area of Science:

  • Clinical Chemistry
  • Immunology
  • Endocrinology

Background:

  • Antibody-based assays are crucial for analyzing clinical analytes but can be susceptible to interference.
  • Endogenous molecules, such as circulating antibodies and free biotin, can interfere with immunoassay accuracy.
  • Discrepancies between laboratory findings and clinical presentation necessitate investigation into assay validity.

Observation:

  • A 14-year-old male presented with critically elevated free thyroxine (fT4) levels, inconsistent with clinical signs of hyperthyroidism.
  • Repeat testing showed discrepant fT4 results across different laboratory platforms.
  • Roche instrumentation yielded critically high fT4, while Siemens Centaur showed normal levels.

Findings:

  • Comprehensive evaluation, including multi-platform testing and serial dilution, was performed.
  • Heterophile antibody interference was identified as the probable cause of aberrant fT4 measurements.
  • The patient's laboratory results were inconsistent due to antibody interference.

Implications:

  • This case underscores the critical need to validate immunoassay results, especially when discordant with clinical data.
  • Awareness of heterophile antibody interference is essential for accurate diagnosis and patient management in endocrinology.
  • Laboratory investigation is vital to identify interference and ensure reliable diagnostic testing.