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In vitro photodynamic inactivation effects of hypocrellin B on azole-sensitive and resistant Candida albicans

Asif Jan1, Chengcheng Liu1, Hong Deng2

  • 1Department of Pathogenic Microbiology & Immunology, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, 76 West Yanta Road, Xi'an, 710061, PR China.

Photodiagnosis and Photodynamic Therapy
|July 21, 2019
PubMed
Abstract

Insights

Hypocrellin B (HB) effectively inactivated azole-sensitive and resistant Candida albicans using antimicrobial photodynamic therapy (aPDT). This light-activated treatment shows promise for combating drug-resistant fungal infections.

Area of Science:

  • Microbiology
  • Photochemistry
  • Medical Mycology

Background:

  • Antifungal drug resistance in Candida albicans necessitates novel therapeutic strategies.
  • Antimicrobial photodynamic therapy (aPDT) offers a promising alternative for localized C. albicans infections.

Purpose of the Study:

  • To evaluate the photodynamic inactivation (PDI) efficacy of hypocrellin B (HB) against azole-sensitive and resistant Candida albicans strains in vitro.

Main Methods:

  • Assessed PDI efficacy of HB on standard, azole-sensitive, and azole-resistant C. albicans.
  • Investigated HB uptake using confocal laser scanning microscopy (CLSM).
  • Examined cellular damage via transmission electron microscopy (TEM) and scanning electron microscopy (SEM).

Main Results:

  • HB demonstrated no dark toxicity but inactivated C. albicans in a light-dose and photosensitizer concentration-dependent manner.
  • PDI treatment induced significant damage to C. albicans cell walls, membranes, and cytoplasm.
  • SEM revealed cell surface twisting and rupture following HB-mediated PDI.

Conclusions:

  • Hypocrellin B effectively inactivates both azole-sensitive and resistant C. albicans in the presence of light.
  • HB-mediated aPDT presents a potential new antifungal treatment for C. albicans infections.
Keywords:
Antimicrobial photodynamic therapyAzole-resistantCandida albicansHypocrellin B

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