Principal Neurons in the Anteroventral Cochlear Nucleus Express Cell-Type Specific Glycine Receptor α Subunits

Signal processing in the principal neurons of the anteroventral cochlear nucleus (AVCN) is modulated by glycinergic inhibition. The kinetics of IPSCs are specific to the target neurons. It remains unclear what glycine receptor subunits are involved in generating such target-specific IPSC kinetics in AVCN principal neurons. We investigated the expression patterns of glycine receptor α (GlyRα) subunits in AVCN using immunohistochemical labeling of four isoforms of GlyRα subunits (GlyRα₁-α₄), and found that AVCN neurons express GlyRα₁ and GlyRα₄, but not GlyRα₂ and GlyRα₃ subunits. To further identify the cell type-specific expression patterns of GlyRα subunits, we combined whole-cell patch clamp recording with immunohistochemistry by recording from all three types of AVCN principal neurons, characterizing the synaptic properties of their glycinergic inhibition, dye-filling the neurons, and processing the slice for immunostaining of different GlyRα subunits. We found that AVCN bushy neurons express both GlyRα₁ and GlyRα₄ subunits that underlie their slow IPSC kinetics, whereas both T-stellate and D-stellate neurons express only GlyRα₁ subunit that underlies their fast IPSC kinetics. In conclusion, AVCN principal neurons express cell-type specific GlyRα subunits that underlie their distinct IPSC kinetics, which enables glycinergic inhibition from the same source to exert target cell-specific modulation of activity to support the unique physiological function of these neurons.