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AMOT130/YAP pathway in topography-induced BMSC osteoblastic differentiation
Xuan Liu1, Wenqing Hou1, Lei He2
1College of Medicine, Southwest Jiaotong University, Chengdu 610031, China.
Colloids and Surfaces. B, Biointerfaces
|July 21, 2019
Summary
Micro/nano-topography on titanium enhances bone marrow mesenchymal stem cell differentiation via the AMOT130/YAP pathway. This pathway translates topographical signals into osteoblastic commitment, crucial for bone biomaterial osseointegration.
Area of Science:
- Biomaterials Science
- Cell Biology
- Tissue Engineering
Background:
- Micro/nano-topography (MNT) influences osseointegration of bone biomaterials.
- The precise mechanisms linking MNT to osteoblastic differentiation remain unclear.
Purpose of the Study:
- To investigate the role of the AMOT130/YAP pathway in osteoblastic differentiation of bone marrow mesenchymal stem cells (BMSCs) cultured on titanium (Ti) with MNTs.
- To elucidate how MNTs on Ti surfaces mediate cellular responses.
Main Methods:
- Preparation of Ti surfaces with defined TiO2 nanotubes (MNTs) via anodization.
- Culture of rat BMSCs on flat Ti and MNT-modified Ti surfaces.
- Analysis of BMSC morphology, F-actin, osteoblastic gene/protein expression, and YAP localization.
Main Results:
- MNT-modified Ti surfaces significantly increased BMSC F-actin formation, osteoblastic gene expression, and AMOT130 protein levels compared to flat Ti.
- Larger nanotube structures showed a more pronounced effect on osteoblastic differentiation.
- Elevating AMOT130 levels enhanced osteoblastic gene expression, F-actin formation, and YAP nuclear localization.
Conclusions:
- The AMOT130/YAP pathway is critical for translating MNT signals into BMSC osteoblastic commitment.
- The mechanism involves AMOT130 promoting F-actin formation, leading to increased YAP nuclear import and subsequent activation of osteoblastic gene expression.
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