Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Constant Volume Calorimetry02:41

Constant Volume Calorimetry

30.6K
Calorimeters are useful to determine the heat released or absorbed by a chemical reaction. Coffee cup calorimeters are designed to operate at constant (atmospheric) pressure and are convenient to measure heat flow (or enthalpy change) accompanying processes that occur in solution at constant pressure. A different type of calorimeter that operates at constant volume, colloquially known as a bomb calorimeter, is used to measure the energy produced by reactions that yield large amounts of heat and...
30.6K
Comparing Copy Number Variations and SNPs02:26

Comparing Copy Number Variations and SNPs

18.6K
Sequencing of the human genome has opened up several best-kept secrets of the genome. Scientists have identified thousands of genome variations that exist within a population. These variations can be a single nucleotide or a larger chromosomal variation.
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...
18.6K
Work Done During Volume Change01:17

Work Done During Volume Change

5.1K
In mechanics, work is done on an object when the force acting on it displaces the object. In thermodynamics, work done on a system can be estimated when the system's volume changes during any thermodynamic process.
Consider a gas confined to a cylinder fitted with a movable piston at one end. If the gas expands from volume V1 to volume V2, it exerts a force on the piston, such that the piston moves by a distance dr.
The work done by the gas on the piston can be expressed as
5.1K
Respiratory Volumes01:15

Respiratory Volumes

2.8K
Respiratory volumes are crucial metrics, meticulously measured to quantify the air exchanged in and out of the lungs during various phases of the breathing cycle. These precise measurements are vital for assessing lung function, diagnosing respiratory conditions, and monitoring overall respiratory health. Each parameter provides specific insights into the mechanics of breathing and the functional capacity of the lungs.
Tidal Volume (TV) Tidal volume (TV) is the air inhaled or exhaled in a...
2.8K
Volume of Distribution01:20

Volume of Distribution

1.2K
The apparent volume of distribution (Vd) is a crucial pharmacokinetic parameter representing the hypothetical body fluid volume into which a drug disperses. It is calculated based on the total amount of drug in the body (estimated from the administered dose and bioavailability) divided by the plasma drug concentration. The total amount of drug in the body does not directly refer to the dose given but is derived by accounting for absorption, distribution, metabolism, and excretion processes.
1.2K
Comparing the Survival Analysis of Two or More Groups01:20

Comparing the Survival Analysis of Two or More Groups

573
Survival analysis is a cornerstone of medical research, used to evaluate the time until an event of interest occurs, such as death, disease recurrence, or recovery. Unlike standard statistical methods, survival analysis is particularly adept at handling censored data—instances where the event has not occurred for some participants by the end of the study or remains unobserved. To address these unique challenges, specialized techniques like the Kaplan-Meier estimator, log-rank test, and...
573

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

From lipofuscin accumulation to cellular dysfunction: a focus on liver pathophysiology.

Histochemistry and cell biology·2026
Same author

Cellular accumulation of lipofuscin in the heart: implications in health and disease.

Histochemistry and cell biology·2026
Same author

Reviving formalin-fixed, paraffin embedded (FFPE) tissues for on-slide and multiscale correlative microscopy.

Scientific reports·2026
Same author

The changing landscape of training in microscopy facilities: Reflections on seven decades of research user support at the University of Sydney.

Journal of microscopy·2026
Same author

MDFIC2 is a sensory neuron-specific PIEZO channel auxiliary subunit.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

A 3D-Printed Pulsatile Shear Stress Platform for Studying Endothelial Cell Mechanobiology.

Analytical chemistry·2026

Related Experiment Video

Updated: Jan 21, 2026

Array Tomography Workflow for the Targeted Acquisition of Volume Information using Scanning Electron Microscopy
09:47

Array Tomography Workflow for the Targeted Acquisition of Volume Information using Scanning Electron Microscopy

Published on: July 15, 2021

5.3K

Expedited large-volume 3-D SEM workflows for comparative microanatomical imaging.

Gerald John Shami1, Delfine Cheng1, Filip Braet2

  • 1School of Medical Sciences (Discipline of Anatomy and Histology)-The Bosch Institute, The University of Sydney, Sydney, NSW, Australia.

Methods in Cell Biology
|July 22, 2019
PubMed
Summary

This paper compares two 3-D scanning electron microscopy (3-D SEM) methods for biological tissue reconstruction: serial-block face/focused ion beam SEM and array tomography. It details workflows and discusses their strengths and limitations for generating 3-D data.

Keywords:
Array tomographyComparative morphomicsCorrelating structureCorrelative microscopyHepaticLarge-volume electron microscopyLiverMicroanatomical structureMultidimensional microscopySectioningTissue arrayZebrafish larvae

More Related Videos

Multimodal Hierarchical Imaging of Serial Sections for Finding Specific Cellular Targets within Large Volumes
11:19

Multimodal Hierarchical Imaging of Serial Sections for Finding Specific Cellular Targets within Large Volumes

Published on: March 20, 2018

10.8K
In Situ Characterization of Boehmite Particles in Water Using Liquid SEM
11:59

In Situ Characterization of Boehmite Particles in Water Using Liquid SEM

Published on: September 27, 2017

9.7K

Related Experiment Videos

Last Updated: Jan 21, 2026

Array Tomography Workflow for the Targeted Acquisition of Volume Information using Scanning Electron Microscopy
09:47

Array Tomography Workflow for the Targeted Acquisition of Volume Information using Scanning Electron Microscopy

Published on: July 15, 2021

5.3K
Multimodal Hierarchical Imaging of Serial Sections for Finding Specific Cellular Targets within Large Volumes
11:19

Multimodal Hierarchical Imaging of Serial Sections for Finding Specific Cellular Targets within Large Volumes

Published on: March 20, 2018

10.8K
In Situ Characterization of Boehmite Particles in Water Using Liquid SEM
11:59

In Situ Characterization of Boehmite Particles in Water Using Liquid SEM

Published on: September 27, 2017

9.7K

Area of Science:

  • Biological imaging
  • Electron microscopy
  • Cellular and tissue analysis

Background:

  • Various electron microscopy (EM) techniques exist for 3-D data acquisition at micro to nanometer scales.
  • Numerous methodologies are available for reconstructing biological volumes.
  • Choosing the optimal workflow for 3-D SEM data generation is crucial.

Purpose of the Study:

  • To outline and compare two distinct specimen manipulation workflows for 3-D scanning electron microscopy (3-D SEM) data generation.
  • To apply and evaluate these workflows using rodent and zebrafish liver as model systems.
  • To provide an in-depth discussion of the strengths and limitations of each 3-D SEM workflow.

Main Methods:

  • Serial-block face/focused ion beam scanning electron microscopy (SBF/FIB-SEM) workflow.
  • Array tomography (AT) workflow.
  • Application to rodent and zebrafish liver tissues.

Main Results:

  • Detailed procedural steps for both SBF/FIB-SEM and AT workflows are presented.
  • Comparative analysis of the strengths and limitations of each method is provided.
  • Demonstration of 3-D data generation capabilities in liver tissues.

Conclusions:

  • Both SBF/FIB-SEM and AT are effective 3-D SEM specimen manipulation workflows for biological research.
  • Understanding the specific advantages and disadvantages of each workflow is essential for selecting the appropriate method.
  • These workflows enable detailed 3-D structural analysis of cells and tissues.