Comet assay genotoxicity evaluation of occupationally exposed tea-garden workers in northern West Bengal, India

To assess possible genotoxic effects of pesticide exposure in occupationally exposed tea-garden workers, DNA damage in peripheral blood lymphocytes was evaluated with the comet assay. The effects of smoking and alcohol consumption were also examined. The comet tail length, % tail DNA, and Olive tail moment were significantly higher in pesticide-exposed workers compared to non-exposed controls. None of the damage parameters differed when control smokers and control alcohol consumers were compared with controls. However, pesticide-exposed individuals had significantly higher comet tail length, % tail DNA, and Olive tail moment than control smokers and control alcohol consumers, suggesting that the DNA damage may be associated with pesticide exposure.