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Screening commercial teat disinfectants against bacteria isolated from bovine milk using disk diffusion
Sarah Rose Fitzpatrick1,2, Mary Garvey2, Kieran Jordan3
1Department of Livestock Systems, Teagasc, Animal and Grassland Research and Innovation Centre, Moorepark, Fermoy, County Cork, Ireland.
Veterinary World
|July 23, 2019
Summary
Combined teat disinfectants effectively inhibit mastitis-causing bacteria. Lactic acid combinations showed superior efficacy, highlighting the importance of ingredient synergy in bovine mastitis control.
Area of Science:
- Veterinary Microbiology
- Dairy Science
- Antimicrobial Efficacy Testing
Background:
- Bovine mastitis poses a significant economic and animal welfare challenge in the dairy industry.
- Effective teat disinfection is crucial for preventing mastitis transmission.
- Identifying specific mastitis-associated bacteria informs the selection of appropriate disinfectants.
Purpose of the Study:
- To evaluate the inhibitory activity of commercial teat disinfectants against mastitis-causing bacteria.
- To determine the efficacy of different disinfectant formulations and ingredient combinations.
Main Methods:
- Twelve commercial teat disinfectant products were tested.
- Disk diffusion assays were employed to assess inhibition zones against 12 mastitis-associated bacterial strains.
- Bacterial isolates were sourced from bulk tank milk, clinical, and subclinical quarter foremilk samples.
Main Results:
- Significant variation in bacterial inhibition (7-30 mm) was observed among the tested disinfectants.
- Products containing lactic acid combined with chlorhexidine or salicylic acid demonstrated the highest levels of bacterial inhibition (p<0.05).
- Combined ingredients exhibited greater inhibitory effects compared to individual components.
Conclusions:
- Synergistic effects of combined ingredients enhance the efficacy of teat disinfection products.
- Lactic acid-based combinations are highly effective against mastitis-associated bacteria.
- The disk diffusion method is a reliable tool for screening and comparing teat disinfectant performance.
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