Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Storage01:23

Storage

369
A schema is a mental framework that helps individuals organize and interpret information. Schemata, formed from previous experiences, influence how we process new information: how we encode it, the inferences we make, and how we retrieve it. For instance, a schema for what a typical classroom looks like might include desks, a teacher's desk, a whiteboard, and students in such an environment. This expectation helps us quickly understand and navigate new classrooms without needing to analyze...
369
Sugars as Energy Storage Molecules01:10

Sugars as Energy Storage Molecules

9.8K
Sugar (a simple carbohydrate) metabolism (chemical reactions) is a classic example of the many cellular processes that use and produce energy. Living things consume sugar as a major energy source because sugar molecules have considerable energy stored within their bonds. Consumed carbohydrates have their origins in photosynthesizing organisms like plants. During photosynthesis, plants use the energy of sunlight to convert carbon dioxide gas into sugar molecules, like glucose. Because this...
9.8K
ATP Energy Storage and Release01:31

ATP Energy Storage and Release

14.0K
ATP is a highly unstable molecule. Unless quickly used to perform work, ATP spontaneously dissociates into ADP and inorganic phosphate (Pi), and the free energy released during this process is lost as heat. The energy released by ATP hydrolysis is used to perform work inside the cell and depends on a strategy called energy coupling. Cells couple the exergonic reaction of ATP hydrolysis with endergonic reactions, allowing them to proceed.
One example of energy coupling using ATP involves a...
14.0K
Fats as Energy Storage Molecules01:06

Fats as Energy Storage Molecules

26.9K
Triglycerides are a form of long-term energy storage molecules. They are made of glycerol and three fatty acids. To obtain energy from fat, triglycerides must first be broken down by hydrolysis into their two principal components, fatty acids and glycerol. This process, called lipolysis, takes place in the cytoplasm. The resulting fatty acids are oxidized by β-oxidation into acetyl-CoA, which is used by the Krebs cycle. The glycerol that is released from triglycerides after lipolysis...
26.9K
Corrosion02:49

Corrosion

28.1K
The degradation of metals due to natural electrochemical processes is known as corrosion. Rust formation on iron, tarnishing of silver, and the blue-green patina that develops on copper are examples of corrosion. Corrosion involves the oxidation of metals. Sometimes it is protective, such as the oxidation of copper or aluminum, wherein a protective layer of metal oxide or its derivatives forms on the surface, protecting the underlying metal from further oxidation. In other cases, corrosion is...
28.1K
Quantifying Heat02:46

Quantifying Heat

61.8K
Thermal Energy Microscopically, thermal energy is the kinetic energy associated with the random motion of atoms and molecules. Temperature is a quantitative measure of “hot” or “cold”, which depends on the amount of thermal energy. When the atoms and molecules in an object are moving or vibrating quickly, they have a higher average kinetic energy (KE) (or higher thermal energy), and the object is perceived as “hot”, or it is described as being at a higher temperature. When the...
61.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Revealing Dentin Multiscale Structures Using High-Resolution Transmission Electron Microscopy.

Journal of dental research·2025
Same author

Unequal Impact of <i>COL1A1</i> and <i>COL1A2</i> Variants on Dentinogenesis Imperfecta.

Journal of dental research·2023
Same author

Transcriptional Regulation of Jaw Osteoblasts: Development to Pathology.

Journal of dental research·2022
Same author

The frequency of interleukin-1β-producing monocytes is significantly associated with varicella-zoster responses of nursing home residents.

Clinical and experimental immunology·2021
Same author

Adapting epicutaneous patch testing protocols to assess immediate-type skin reactions.

International journal of cosmetic science·2020
Same author

Exogenous interleukin-2 can rescue in-vitro T cell activation and proliferation in patients with a novel capping protein regulator and myosin 1 linker 2 mutation.

Clinical and experimental immunology·2020

Related Experiment Video

Updated: Jan 21, 2026

Quantitating Iron Transport Across the Mouse Placenta In Vivo Using Nonradioactive Iron Isotopes
08:45

Quantitating Iron Transport Across the Mouse Placenta In Vivo Using Nonradioactive Iron Isotopes

Published on: May 10, 2022

2.4K

Disrupted Iron Storage in Dental Fluorosis.

S Houari1,2, E Picard3, T Wurtz1

  • 11 Centre de Recherche des Cordeliers, INSERM UMRS 1138, Université de Paris, Sorbonne Université, Laboratory of Molecular Oral Pathophysiology, Paris, France.

Journal of Dental Research
|July 23, 2019
PubMed
Summary

Excessive fluoride exposure impairs iron storage in ameloblasts, leading to defective enamel structure and mechanical properties in mice. This highlights potential impacts on broader health.

Keywords:
ameloblastsdental enamelferritinsfluoridesfluorosisgene expression

More Related Videos

Measurement of Tissue Non-Heme Iron Content using a Bathophenanthroline-Based Colorimetric Assay
05:08

Measurement of Tissue Non-Heme Iron Content using a Bathophenanthroline-Based Colorimetric Assay

Published on: January 31, 2022

5.5K
Isolation of Epithelial Cells from Human Dental Follicle
04:07

Isolation of Epithelial Cells from Human Dental Follicle

Published on: November 5, 2021

3.2K

Related Experiment Videos

Last Updated: Jan 21, 2026

Quantitating Iron Transport Across the Mouse Placenta In Vivo Using Nonradioactive Iron Isotopes
08:45

Quantitating Iron Transport Across the Mouse Placenta In Vivo Using Nonradioactive Iron Isotopes

Published on: May 10, 2022

2.4K
Measurement of Tissue Non-Heme Iron Content using a Bathophenanthroline-Based Colorimetric Assay
05:08

Measurement of Tissue Non-Heme Iron Content using a Bathophenanthroline-Based Colorimetric Assay

Published on: January 31, 2022

5.5K
Isolation of Epithelial Cells from Human Dental Follicle
04:07

Isolation of Epithelial Cells from Human Dental Follicle

Published on: November 5, 2021

3.2K

Area of Science:

  • Biomineralization
  • Dental Enamel Formation
  • Fluoride Toxicology

Background:

  • Enamel quality depends on environmental factors, including fluoride exposure.
  • Excess fluoride can alter enamel structure and gene expression.
  • Iron is crucial for enamel quality.

Purpose of the Study:

  • To investigate iron metabolism in dental epithelial cells and forming enamel of fluoride-exposed mice.
  • To assess the relationship between fluoride exposure, iron metabolism, and enamel mechanical properties.

Main Methods:

  • Perl's blue staining and secondary ion mass spectrometry imaging for iron storage.
  • Quantitative real-time polymerase chain reaction, Western blotting, and immunohistochemistry for protein analysis.
  • Electron spin resonance, nanoindentation, scanning electron microscopy, and energy dispersive x-ray spectroscopy for enamel analysis.
  • In vivo studies using Fth+/- mice.

Main Results:

  • Fluoride exposure significantly reduced iron storage in maturation-stage ameloblasts.
  • Ferritin heavy chain (Fth) was identified as a primary target of fluoride.
  • Fluorotic enamel showed decreased iron oxides, altered mechanical properties, and ultrastructural defects.
  • Fth+/- mice exhibited reduced iron incorporation and poor enamel quality.

Conclusions:

  • Excessive fluoride exposure disrupts ameloblast iron storage, contributing to defective enamel.
  • Fluoride-induced alterations in iron metabolism impact enamel's structural and mechanical integrity.
  • Findings suggest potential implications of fluoride's effects on iron storage for population health.