Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Excitation-Contraction Coupling in Skeletal Muscles01:20

Excitation-Contraction Coupling in Skeletal Muscles

14.4K
Excitation-contraction coupling is a series of events that occur between generating an action potential and initiating a muscle contraction. It occurs at the triad, a structure found in skeletal muscle fibers that comprise a T-tubule and terminal cisternae of the sarcoplasmic reticulum on each side. These triads are visible in longitudinally sectioned muscle fibers. They are typically located at the A-I junction — the junction between the A and I bands of the sarcomere.
When an action...
14.4K
Difference from Background: Limit of Detection01:05

Difference from Background: Limit of Detection

8.1K
The limit of detection (LOD) is the smallest amount of analyte that can be distinguished from the background noise. The LOD value corresponds to the concentration at which the analyte signal is three times larger than the standard deviation of the blank signal. Below this value, the analyte signal cannot be differentiated from the background noise. It is calculated by dividing the calibration slope by 3 times the standard deviation of the blank signals.
The LOD indicates the presence or absence...
8.1K
Electric Potential and Potential Difference01:16

Electric Potential and Potential Difference

5.6K
Suppose a positive test charge moves away from a positive static charge, then the Coulomb force does positive work, and its electric potential energy decreases. The potential energy per unit charge is defined as the electric potential. The electric potential is independent of the test charge.
When a test charge moves from the initial to the final position, the electric potential difference between those positions is defined as the ratio of the change in the potential energy to the charge on the...
5.6K
Identifying Statistically Significant Differences: The F-Test01:14

Identifying Statistically Significant Differences: The F-Test

3.2K
The F-test is used to compare two sample variances to each other or compare the sample variance to the population variance. It is used to decide whether an indeterminate error can explain the difference in their values. The underlying assumptions that allow the use of the F-test include the data set or sets are normally distributed, and the data sets are independent of each other. The test statistic F is calculated by dividing one variance by another. In other words, the square of one standard...
3.2K
Sum and Difference OpAmps01:22

Sum and Difference OpAmps

1.3K
Operational amplifiers (op-amps) are versatile devices that extend beyond amplification. In this context, two specific op-amp configurations are explored: the summing and difference amplifiers.
A summing amplifier, or an adder, utilizes an op-amp to merge multiple input signals into a single output signal. When audio signals are introduced into its input channels, the input resistors initiate currents that traverse feedback resistors, resulting in an output voltage. Applying Kirchhoff's current...
1.3K
Difference Equation Solution using z-Transform01:24

Difference Equation Solution using z-Transform

625
The z-transform is a powerful tool for analyzing practical discrete-time systems, often represented by linear difference equations. Solving a higher-order difference equation requires knowledge of the input signal and the initial conditions up to one term less than the order of the equation.
The z-transform facilitates handling delayed signals by shifting the signal in the z-domain, which corresponds to delaying the signal in the time domain, and advancing signals by similarly shifting in the...
625

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Targeted long-read sequencing enables comprehensive analysis of the genetic and epigenetic landscape of inherited myopathies.

Nature communications·2026
Same author

Reduced electroencephalogram power and no change in peak alpha frequency in individuals with chronic migraine: a cross-sectional investigation.

Neurophysiologie clinique = Clinical neurophysiology·2026
Same author

Glioblastoma mimicking anti-NMDA receptor encephalitis: a case series.

Internal medicine journal·2026
Same author

MRI-measured tendon retraction distance is associated with EMG-confirmed neurotrauma in proximal hamstring avulsion.

Journal of experimental orthopaedics·2026
Same author

A Study of Time to Recovery Following Loss of Neuromonitoring Signal of the Recurrent Laryngeal Nerve in Thyroid Surgery.

World journal of surgery·2026
Same author

Timing matters when using paraclinical tests to assess for optic neuritis under the 2024 McDonald criteria.

BMJ neurology open·2026

Related Experiment Video

Updated: Jan 21, 2026

Author Spotlight: Functional Site-Directed Fluorometry in Native Cells to Study Skeletal Muscle Excitability
12:26

Author Spotlight: Functional Site-Directed Fluorometry in Native Cells to Study Skeletal Muscle Excitability

Published on: June 2, 2023

1.4K

Physiological differences in sarcolemmal excitability between human muscles.

James H F Lee1, Robert Boland-Freitas1, Karl Ng1

  • 1Neurology Department, Royal North Shore Hospital, St Leonards, New South Wales, Australia.

Muscle & Nerve
|July 23, 2019
PubMed
Summary

Human muscle resting membrane potential (RMP) varies by muscle type. This study found differences in RMP between the rectus femoris and tibialis anterior muscles, highlighting the need for muscle-specific data.

Keywords:
excitabilitymuscle fiber typemuscle velocity recovery cyclesresting membrane potentialskeletal muscle

More Related Videos

Historical View and Physiology Demonstration at the NMJ of the Crayfish Opener Muscle
11:56

Historical View and Physiology Demonstration at the NMJ of the Crayfish Opener Muscle

Published on: November 9, 2009

21.9K
Physiological Recordings of High and Low Output NMJs on the Crayfish Leg Extensor Muscle
10:00

Physiological Recordings of High and Low Output NMJs on the Crayfish Leg Extensor Muscle

Published on: November 17, 2010

12.1K

Related Experiment Videos

Last Updated: Jan 21, 2026

Author Spotlight: Functional Site-Directed Fluorometry in Native Cells to Study Skeletal Muscle Excitability
12:26

Author Spotlight: Functional Site-Directed Fluorometry in Native Cells to Study Skeletal Muscle Excitability

Published on: June 2, 2023

1.4K
Historical View and Physiology Demonstration at the NMJ of the Crayfish Opener Muscle
11:56

Historical View and Physiology Demonstration at the NMJ of the Crayfish Opener Muscle

Published on: November 9, 2009

21.9K
Physiological Recordings of High and Low Output NMJs on the Crayfish Leg Extensor Muscle
10:00

Physiological Recordings of High and Low Output NMJs on the Crayfish Leg Extensor Muscle

Published on: November 17, 2010

12.1K

Area of Science:

  • Human physiology
  • Skeletal muscle electrophysiology

Background:

  • Sarcolemmal resting membrane potential (RMP) influences muscle function.
  • Limited in vivo data exists for human RMP across different muscles.

Purpose of the Study:

  • To investigate potential differences in in vivo RMP between human muscles with distinct physiological roles.
  • To compare the RMP of a proximal antigravity muscle (rectus femoris) with a distal non-antigravity muscle (tibialis anterior).

Main Methods:

  • Muscle velocity recovery cycles were measured in vivo.
  • Recordings were obtained from the rectus femoris and tibialis anterior muscles in 34 healthy individuals.

Main Results:

  • Significant differences were observed in the muscle relative refractory period, early supernormality, and late supernormality between the two muscles.
  • These electrophysiological differences suggest a less negative RMP in the tibialis anterior compared to the rectus femoris.

Conclusions:

  • The findings indicate inter-muscle variations in normal human skeletal muscle excitability and physiology.
  • This study underscores the necessity of establishing muscle-specific normative data for RMP and related electrophysiological parameters.