Identification of a functional nuclear localization signal in 3D^pol/3CD of duck hepatitis A virus 1

The nuclear localization signals (NLS) were usually composed of basic residues (K and R) and played an important role in delivery of genomes and structural protein into nucleus. In this research, we identified that 3D^pol/3CD entered into nucleus during viral propagation of duck hepatitis A virus type 1 (DHAV-1). To investigate the reason that 3D^pol/3CD entered into nucleus, the amino acid sequence of 3CD was analyzed through NLS Mapper program. The basic region ¹⁷PRKTAYMRS²⁵ was subsequently proved to be a functional NLS to guide 3D^pol/3CD into nucleus. ¹⁸R, ¹⁹K and ²⁴R were found essential for maintaining the nuclear targeting activity, and exchange between ²⁴R and ²⁴K had no impact on cellular localization of 3D^pol. Since the entry of 3D^pol/3CD into nucleus was essential for shutoff of host cell transcription and maintaining the viral propagation of picornavirus numbers, our study provided new insights into the mechanism of DHAV-1 propagation.