Optical Temperature Control Unit and Convolutional Neural Network for Colorimetric Detection of Loop-Mediated...

Area of Science:

• Biomedical Engineering
• Microfluidics
• Molecular Diagnostics

Background:

• Lab-on-a-disc (LOD) platforms offer potential for point-of-care testing (POCT) due to integrated fluid handling.
• Non-contact temperature measurement and heating are critical challenges for LOD-based DNA amplification, especially on rotating discs.

Purpose of the Study:

• To develop an automatic Lab-on-a-disc (LOD) system for loop-mediated isothermal amplification (LAMP).
• To overcome the limitations of contact-based temperature control in LOD devices for DNA amplification.

Main Methods:

• Integrated a thermochromic coating for non-contact temperature measurement (<~420 µm).
• Incorporated a micro graphite film for efficient remote laser absorption and heating.
• Utilized a deep learning network for enhanced analysis of LAMP products.

Main Results:

• Demonstrated effective non-contact temperature heating and measurement on the LOD surface.
• Achieved highly efficient loop-mediated isothermal amplification (LAMP) within the LOD system.
• Showcased superior analysis of LAMP products using deep learning compared to visual inspection.

Conclusions:

• The developed LOD-based system successfully integrates non-contact temperature control and analysis for isothermal DNA amplification.
• This approach provides a robust and efficient solution for advanced point-of-care diagnostic devices.
• The study presents a novel method for remote thermal management in microfluidic devices.