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Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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c-fos expression interferes with thymus development in transgenic mice.

U Rüther1, W Müller, T Sumida

  • 1European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany.

Cell
|June 17, 1988
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The proto-oncogene c-fos, when overexpressed in transgenic mice, leads to enlarged spleens and thymuses, impacting T cell development and causing immune deficiency. This suggests c-fos indirectly affects T cell maturation by stimulating thymic epithelial cells.

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Area of Science:

  • Immunology
  • Oncology
  • Developmental Biology

Background:

  • Proto-oncogenes like c-fos play critical roles in cell growth and differentiation.
  • Understanding c-fos function in hematopoietic tissues is crucial for comprehending immune system development and potential oncogenesis.

Purpose of the Study:

  • To investigate the role of c-fos proto-oncogene in hematopoietic tissues using transgenic mouse models.
  • To determine the effects of exogenous c-fos expression on T cell development and immune function.

Main Methods:

  • Generation of transgenic mice (H2-c-fos) expressing c-fos under the H2-Kb promoter.
  • Analysis of spleen and thymus morphology, cell populations, and hematopoietic lineages.
  • Utilizing bone marrow radiation chimeras to assess cell-specific effects.

Main Results:

  • H2-c-fos mice exhibited enlarged spleens and hyperplastic thymuses with increased thymic epithelial cells.
  • Exogenous c-fos expression altered T cell development, increasing mature thymocytes but impairing B and T cell function, leading to immune deficiency.
  • Altered T cell subset distribution was not a direct effect of c-fos in T cells, suggesting indirect mechanisms.

Conclusions:

  • c-fos overexpression in transgenic mice leads to thymic epithelial cell proliferation, indirectly impacting T cell development.
  • The study reveals a complex role for c-fos in immune regulation, highlighting its potential to induce immune deficiency rather than malignancy in this context.