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p62/SQSTM1 expression in canine mammary tumours: Evolutionary notes
Francesca Mariotti1, Gian Enrico Magi1, Alessandra Gavazza1
1School of Biosciences and Veterinary Medicine, University of Camerino, Italy.
Veterinary and Comparative Oncology
|July 24, 2019
Summary
Autophagy protein p62 (Sequestosome1) expression differs between canine and human cancers. Canine mammary tumors show lower p62 in carcinomas, unlike human breast cancer, impacting immunotherapy potential.
Area of Science:
- Cell Biology
- Oncology
- Veterinary Medicine
Background:
- Autophagy is crucial for homeostasis and cancer signaling.
- p62/SQSTM1, an autophagy receptor, is implicated in tumor development and inflammation.
- Preclinical models for p62-based cancer medicine lack comparative investigation.
Purpose of the Study:
- To investigate the role of p62 in canine mammary tumors.
- To compare canine p62 expression with human and other animal sequences.
- To evaluate p62 as a potential target for canine cancer immunotherapy.
Main Methods:
- Comparative sequence analysis of p62 across species.
- Immunohistochemical analysis of p62 expression in 66 canine mammary tumors and 10 non-neoplastic samples.
- Correlation of p62 levels with tumor grade and type.
Main Results:
- Canine p62 sequences show significant divergence from human and other animal sequences.
- p62 expression was higher in normal canine mammary tissue and adenomas than in carcinomas.
- Lowest p62 protein levels were observed in high-grade canine mammary carcinomas, with negative stromal expression.
Conclusions:
- The association between p62 expression and cancer in dogs is inverse to that in human breast carcinoma.
- p62 accumulation in malignant cells, seen in humans, is not observed in canine mammary tumors.
- p62 is unlikely to be a tumor-rejection antigen for anti-cancer immunotherapy in canine mammary tumors.
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