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CometChip: A High-throughput 96-Well Platform for Measuring DNA Damage in Microarrayed Human Cells
Published on: October 18, 2014
An integrated platform for a high-throughput pharmacokinetic study of glycosides using a boronic acid-functionalized
Ningning Zhao1, Qianqian Gu, Zhiqiang Liu
1National Center of Mass Spectrometry in Changchun and Jilin Province Key Laboratory of Chinese Medicine Chemistry and Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, China. mslab20@ciac.ac.cn.
Abstract:
The novel Vial@FPBA strategy was established for a large-scale pharmacokinetic study of glycosides, during which glycosides were absorbed into a boronic acid-functionalized 96-well glass plate and directly desorbed for UHPLC-MS/MS analysis. Hence, specific and high-throughput glycoside enrichment was achieved simultaneously. The LODs were reduced up to 50 times compared to the case of the methanol method. Meanwhile, sample pre-processing time was greatly saved by skipping the protein sedimentation and supernatant concentration steps.
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