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Fluorescent Probes for the Visualization of Cell Viability.
Minggang Tian1, Yanyan Ma1, Weiying Lin1,2
1Institute of Fluorescent Probes for Biological Imaging, School of Chemistry and Chemical Engineering, School of Materials Science and Engineering , University of Jinan , Jinan , Shandong 250022 , P. R. China.
Accounts of Chemical Research
|July 24, 2019
Summary
This study reviews fluorescent probes for monitoring cell viability, focusing on novel dual-color and reversible designs for accurate apoptosis and necrosis detection in drug discovery.
Area of Science:
- Biochemistry and Molecular Biology
- Cell Biology
- Chemical Biology
Background:
- Cell viability monitoring is critical for apoptosis, necrosis, and drug discovery research.
- Abnormal cell death is linked to diseases like cancer.
- Changes in intracellular contents and physical properties signal cell death.
Purpose of the Study:
- To summarize and classify fluorescent probes for cell viability detection.
- To discuss probe design principles, mechanisms, and characteristics.
- To highlight recent advancements in dual-color and reversible fluorescent probes.
Main Methods:
- Classification of fluorescent probes based on biological changes during cell death.
- Analysis of probe design strategies, fluorescence mechanisms (ESIPT, ICT), and molecular construction.
- Synthesis and application of novel dual-color and reversible probes targeting esterase activity, mitochondrial membrane potential, and membrane integrity.
Main Results:
- Developed dual-color probes based on esterase activity loss, utilizing ESIPT and ICT mechanisms.
- Engineered reversible probes based on mitochondrial membrane potential (ΔΨm) depolarization and relocalization.
- Created simultaneous dual-color and reversible probes using relocalization and aggregation-induced emission shift.
Conclusions:
- Fluorescent probes are powerful tools for studying cell viability, apoptosis, and necrosis.
- Dual-color and reversible probes offer enhanced accuracy and dynamic detection capabilities.
- These advanced probes can significantly advance cell death studies and drug discovery.
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