Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phosphorylation01:02

Phosphorylation

53.7K
The addition or removal of phosphate groups from proteins is the most common chemical modification that regulates cellular processes. These modifications can affect the structure, activity, stability, and localization of proteins within cells as well as their interactions with other proteins.
During phosphorylation, protein kinases transfer the terminal phosphate group of ATP to specific amino acid side chains of substrate proteins. Serine, threonine, and tyrosine are the most commonly...
53.7K
Properties of Enantiomers and Optical Activity02:24

Properties of Enantiomers and Optical Activity

21.5K
It is essential to understand the difference between chiral and achiral interactions and the implications thereof in optical activity and their applications. Just as our feet, which are chiral, interact uniquely with chiral objects, such as a pair of shoes, but identically with achiral socks, enantiomers of a molecule exhibit different properties only when they interact with other chiral media. An example of a significant implication from this facet is the phenomenon known as optical activity,...
21.5K
Glucose Homeostasis: Regulation of Blood Glucose01:02

Glucose Homeostasis: Regulation of Blood Glucose

3.9K
Carbohydrates consumed through foods are converted into glucose, a crucial energy source for the body. In the prandial state, high blood glucose levels stimulate the secretion of insulin from the pancreas. Insulin inhibits hepatic glucose production and stimulates glucose uptake and metabolism by muscle and adipose tissue. The excess glucose is converted into glycogen and stored in the liver and muscles.
During fasting, when blood glucose levels are low, the pancreas secretes glucagon. it...
3.9K
Glucose Transporters01:27

Glucose Transporters

27.2K
Glucose transporters facilitate the transport of glucose across the cell membrane. In addition to glucose, some glucose transporters can also aid the movement of other hexoses such as fructose, mannose, and galactose.
Facilitated diffusion-glucose transporters (GLUTs) are encoded by the solute-linked carrier (SLC) family 2, subfamily A gene family, or SLC2A. The 14 GLUT protein members are distributed into three classes:
27.2K
Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

1.5K
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
1.5K
Glucose Absorption Into the Small Intestine01:26

Glucose Absorption Into the Small Intestine

35.2K
Complex carbohydrates consumed cannot be absorbed into the small intestine in their original form. First, they must be hydrolyzed to a monosaccharide form such as glucose or galactose. These monosaccharides are then transported across the intestinal membrane and into the blood via transcellular transport. The intestinal epithelial cells allow the movement of these monosaccharides with a defined 'entry' through membrane transporter proteins present on their apical membrane and...
35.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Modulating the Electron Mediators for Spatially Separated H<sub>2</sub> and O<sub>2</sub> Evolutions in Photocatalytic Water Splitting.

Angewandte Chemie (International ed. in English)·2026
Same author

Biomimetic Redox-Mediated Proton Relay in Nanoreactors for Photocatalysis.

Journal of the American Chemical Society·2026
Same author

Unraveling the Kinetic Role of Doping in the Oxygen Evolution Reaction on Ce-Mn<sub>3</sub>O<sub>4</sub> Electrocatalysts.

The journal of physical chemistry letters·2026
Same author

Reconfiguration of d-orbital states drives non-radiative energy dissipation in semiconductors.

Materials horizons·2026
Same author

A Theoretical Understanding of both Activity and Stability Promotion of NiFe-Based OER Catalysts via 3d-2p-4f Orbital Hybridization.

The journal of physical chemistry letters·2026
Same author

Spatial imaging of water oxidation on single-particle catalysts.

Nature nanotechnology·2026

Related Experiment Video

Updated: Jan 21, 2026

Optical Trapping of Plasmonic Nanoparticles for In Situ Surface-Enhanced Raman Spectroscopy Characterizations
06:19

Optical Trapping of Plasmonic Nanoparticles for In Situ Surface-Enhanced Raman Spectroscopy Characterizations

Published on: June 23, 2022

2.9K

Stereostructural Elucidation of Glucose Phosphorylation by Raman Optical Activity.

Yuxuan Tang1,2, Feng Cheng1, Zhaochi Feng1

  • 1State Key Laboratory of Catalysis, Dalian Institute of Chemical Physics , Chinese Academy of Sciences , Zhongshan Road 457 , Dalian 116023 , China.

The Journal of Physical Chemistry. B
|July 24, 2019
PubMed
Summary

Raman optical activity (ROA) reveals subtle stereochemical changes during glucose phosphorylation. This technique differentiates glucose 6-phosphate (G6P) and α-glucose 1-phosphate (αG1P) based on phosphorylation sites and conformational shifts.

More Related Videos

Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag
08:55

Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag

Published on: December 14, 2017

16.0K
A High Resolution Method to Monitor Phosphorylation-dependent Activation of IRF3
11:44

A High Resolution Method to Monitor Phosphorylation-dependent Activation of IRF3

Published on: January 24, 2016

12.5K

Related Experiment Videos

Last Updated: Jan 21, 2026

Optical Trapping of Plasmonic Nanoparticles for In Situ Surface-Enhanced Raman Spectroscopy Characterizations
06:19

Optical Trapping of Plasmonic Nanoparticles for In Situ Surface-Enhanced Raman Spectroscopy Characterizations

Published on: June 23, 2022

2.9K
Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag
08:55

Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag

Published on: December 14, 2017

16.0K
A High Resolution Method to Monitor Phosphorylation-dependent Activation of IRF3
11:44

A High Resolution Method to Monitor Phosphorylation-dependent Activation of IRF3

Published on: January 24, 2016

12.5K

Area of Science:

  • Biochemistry
  • Spectroscopy
  • Chirality

Background:

  • Glucose phosphorylation is a critical step in metabolism and energy storage.
  • Key intermediates include glucose 6-phosphate (G6P) and α-glucose 1-phosphate (αG1P).
  • Understanding the stereochemical changes during phosphorylation is vital for enzyme-mediated reactions.

Purpose of the Study:

  • To use Raman optical activity (ROA) to directly observe conformational changes during glucose phosphorylation.
  • To elucidate the stereochemical evolution from glucose to G6P and αG1P.
  • To establish a structural basis for understanding sugar phosphorylation through chirality.

Main Methods:

  • Raman optical activity (ROA) spectroscopy was employed.
  • Analysis focused on characteristic ROA signals related to phosphate group vibrations and rotamer distributions.
  • Conformational flexibility changes were assessed based on ROA spectral features.

Main Results:

  • ROA distinguished between G6P and αG1P based on phosphorylation sites (C6 vs. C1).
  • A (+)980 cm-1 ROA marker indicated phosphorylation at the chiral C1 of αG1P.
  • ROA revealed distinct rotameric populations (gg/gt) and reduced conformational flexibility upon phosphorylation.

Conclusions:

  • ROA is highly sensitive to phosphorylation sites and stereochemical changes in glucose derivatives.
  • The study provides direct evidence of conformational evolution during glucose phosphorylation.
  • ROA offers a powerful tool for investigating chirality in biological molecules.