A novel approach to improving hybrid capture sequencing targeting efficiency
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Updated: Jan 21, 2026

Comparative Lesions Analysis Through a Targeted Sequencing Approach
Published on: November 5, 2019
WenXiang Lu1, Miao Zhu2, Yi Chen3
1Department of R&D, Decode Genomics Incorporation, Nanjing, 210000, China; Department of R&D, AGCU ScienTech Incorporation, Wuxi, 214174, China.
Abstract:
At present, approaches to hybrid capture sequencing have many limitations, from their significant complexity and labor requirements, to their low enrichment efficiency, resulting in their limited utilization. In an effort to overcome these drawbacks, we have developed a novel method that relied upon direct genomic DNA hybridization and single-stranded DNA library preparation. Using this novel protocol, we were able to achieve a targeting efficiency as high as 75%, and we found this approach to overall be an efficient and simple approach to DNA library construction.
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