Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

9.8K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
9.8K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

3.5K
3.5K
Epigenetic Regulation01:46

Epigenetic Regulation

33.5K
Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
33.5K
Epigenetic Regulation01:37

Epigenetic Regulation

3.7K
Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
3.7K
tRNA Activation02:26

tRNA Activation

22.7K
Aminoacyl-tRNA synthetases are present in both eukaryotes and bacteria. Though eukaryotes have 20 different aminoacyl-tRNA synthetases to couple to 20 amino acids, many bacteria do not have genes for all of these aminoacyl-tRNA synthetases. Despite this, they still use all 20 amino acids to synthesize their proteins. For instance, some bacteria do not have the gene encoding the enzyme that couples glutamine with its partner tRNA. In these organisms, one enzyme adds glutamic acid to all of the...
22.7K
Negative Regulator Molecules01:23

Negative Regulator Molecules

38.3K
Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
38.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

iPSC-Derived iNK Progenitors Engraft and Generate NK Cells in Unconditioned and Autologous Immune Humanized Mice.

Cell proliferation·2026
Same author

Bacillus sp. Z26 suppresses Alternaria alternata and its mycotoxins by targeting AaPex13-mediated peroxisome biogenesis.

World journal of microbiology & biotechnology·2026
Same author

Unraveling material characteristics of different pork belly cuts from raw to dish: a comparative study of texture and flavor.

NPJ science of food·2026
Same author

Sodium Alginate/Chitosan/Activated Carbon Composite Hydrogel for Cyanobacterial Inhibition: RSM Optimization and Sustained Release Performance.

Gels (Basel, Switzerland)·2026
Same author

Beneficial Effects of Spermidine on Ovarian Function, Gut Microbiota Composition, and Associated Metabolic Changes.

Nutrients·2026
Same author

Risk factors and prediction model for necrotizing enterocolitis in preterm infants with gestational age ≤ 32 weeks: a retrospective cohort study.

Frontiers in pediatrics·2026

Related Experiment Video

Updated: Jan 21, 2026

Epigenetic Regulation of Cardiac Differentiation of Embryonic Stem Cells and Tissues
13:03

Epigenetic Regulation of Cardiac Differentiation of Embryonic Stem Cells and Tissues

Published on: June 3, 2016

8.6K

tRNA-Derived Small Non-Coding RNAs as Novel Epigenetic Molecules Regulating Adipogenesis.

Linyuan Shen1,2, Zhendong Tan1,2, Mailin Gan1,2

  • 1College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.

Biomolecules
|July 25, 2019
PubMed
Summary

tRNA-derived fragments (tRFs) regulate gene expression. This study identified tRFGluTTC as a key molecule that promotes fat cell proliferation and suppresses differentiation, offering a new strategy for obesity intervention.

Keywords:
3T3-L1KLF familyadipogenesistRFstranscriptome

More Related Videos

Metabolic Labeling and Profiling of Transfer RNAs Using Macroarrays
10:56

Metabolic Labeling and Profiling of Transfer RNAs Using Macroarrays

Published on: January 16, 2018

6.1K
Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms
05:12

Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms

Published on: February 2, 2024

1.3K

Related Experiment Videos

Last Updated: Jan 21, 2026

Epigenetic Regulation of Cardiac Differentiation of Embryonic Stem Cells and Tissues
13:03

Epigenetic Regulation of Cardiac Differentiation of Embryonic Stem Cells and Tissues

Published on: June 3, 2016

8.6K
Metabolic Labeling and Profiling of Transfer RNAs Using Macroarrays
10:56

Metabolic Labeling and Profiling of Transfer RNAs Using Macroarrays

Published on: January 16, 2018

6.1K
Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms
05:12

Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms

Published on: February 2, 2024

1.3K

Area of Science:

  • Molecular Biology
  • Epigenetics
  • RNA Biology

Background:

  • Non-coding RNAs, including tRNA-derived fragments (tRFs), regulate gene expression post-transcriptionally.
  • The role of tRFs in regulating fat deposition and adipogenesis remains largely unexplored.

Purpose of the Study:

  • To investigate the mechanism by which tRFs influence adipogenesis and fat deposition.
  • To identify specific tRFs involved in obesity using a high-fat diet-induced rat model.

Main Methods:

  • Induction of obesity in a rat model using a high-fat diet.
  • tRFs transcriptome sequencing to identify differentially expressed tRFs.
  • Luciferase activity assays to determine direct gene targets of tRFs.

Main Results:

  • Identified 296 differentially expressed tRFs in response to obesity, with tRFGluTTC showing the highest fold change.
  • tRFGluTTC promoted preadipocyte proliferation and suppressed differentiation, reducing triglyceride content and lipid accumulation.
  • tRFGluTTC directly targeted and suppressed Kruppel-like factors (KLF9, KLF11, KLF12) and adipogenic transcription factors (aP2, PPARγ, C/EBPα).

Conclusions:

  • tRFGluTTC acts as a novel epigenetic regulator of adipogenesis.
  • tRFGluTTC may serve as a potential therapeutic target for obesity intervention.