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lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

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In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
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RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
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Long Non-Coding RNA Function in CD4+ T Cells: What We Know and What Next?

Katie A West1,2,3, Dimitris Lagos4,5

  • 1York Biomedical Research Institute, University of York, Wentworth Way, York YO10 5DD, UK.

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Long non-coding RNAs (lncRNAs) are key regulators in T helper cell function. Understanding lncRNA mechanisms offers new therapeutic targets for CD4+ cell-mediated diseases.

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Area of Science:

  • Genomics
  • Immunology
  • Molecular Biology

Background:

  • The non-coding genome, once termed "junk" DNA, is increasingly recognized for its role in mammalian biological complexity.
  • Long non-coding RNAs (lncRNAs) are implicated in diverse cellular functions, including gene regulation, chromosome organization, and splicing.

Purpose of the Study:

  • To investigate the mechanistic role of lncRNAs in T helper cell differentiation, activation, and function.
  • To identify potential therapeutic targets for CD4+ cell-mediated pathologies based on lncRNA function.

Main Methods:

  • Knockdown studies to assess lncRNA function.
  • RNA interaction studies to elucidate molecular mechanisms.
  • Analysis of T helper cell responses upon antigenic stimulation.

Main Results:

  • Several lncRNAs have been identified as crucial for T helper cell differentiation, activation, and function.
  • lncRNAs play significant roles in the finely regulated gene expression changes within T helper cells.

Conclusions:

  • lncRNAs are vital components of T helper cell biology.
  • Further research into lncRNAs in T helper cells may reveal novel therapeutic strategies for CD4+ mediated diseases.