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Updated: Jan 21, 2026

Serum and Plasma Copy Number Detection Using Real-time PCR
Published on: December 15, 2017
LAMP real-time turbidity detection for fowl adenovirus
Xiao-Yuan Yuan1, You-Ling Wang2, Kai Meng3
1Poultry Institute, Shandong Academy of Agricultural Sciences; Shandong Provincial Key Laboratory of Poultry Diseases Diagnosis and Immunology; Poultry Breeding Engineering Technology Center of Shandong Province, Ji'nan, China. xyyuan1980@163.com.
Background:
Fowl adenovirus (FAdV) is an infectious agent that can cause jaundice, severe anaemia, dyspnoea and reproductive disorders in fowls. Since 2015, FAdV disease has been rapidly spreading among broiler chickens in China, where it has caused significant economic losses. In this study, a loop-mediated isothermal amplification (LAMP) real-time turbidity method with strong specificity to FAdV was established.
Results:
The established assay was specific to FAdV-4, and the lower limit of detection was 75 copies/μL of extracted DNA. The positive detection rate for the suspected tissue samples was 33.3% (14/42), which was consistent with that of the real-time PCR.
Conclusion:
The proposed LAMP method can quickly and accurately detect prevalent FAdV via real-time turbidity assay, thereby providing a diagnostic platform for the prevention and control of the FAdV disease.
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