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Protein synthesis and secretion by activated human lymphocytes.
International Archives of Allergy and Applied Immunology
|January 1, 1979
Summary
Human peripheral blood mononuclear cells increase protein synthesis and secretion when stimulated. This response, particularly with concanavalin A (Con A), primarily reflects T-lymphocyte activity and proliferation.
Area of Science:
- Immunology
- Cell Biology
Background:
- Peripheral blood mononuclear cells (PBMCs) are crucial components of the immune system.
- Mitogens like concanavalin A (Con A) are used to stimulate lymphocyte proliferation and function.
Purpose of the Study:
- To investigate the cellular source of protein synthesis and secretion in mitogen-stimulated PBMCs.
- To determine if protein secretion correlates with cellular proliferation in PBMCs.
Main Methods:
- Human PBMCs were stimulated with mitogens, including Con A.
- Protein synthesis and secretion were measured.
- Cellular proliferation was assessed.
- Selective depletion of B lymphocytes and monocytes was performed.
Main Results:
- PBMCs showed increased protein synthesis and secretion upon mitogen stimulation.
- Protein secretion in response to Con A correlated with cellular proliferation.
- The secreted protein originated from proliferating cells.
- Depletion of B cells or monocytes did not significantly reduce total protein secretion.
Conclusions:
- T lymphocytes are the primary contributors to protein synthesis and secretion in Con A-stimulated PBMCs.
- Measuring newly synthesized and secreted protein by Con A-activated PBMCs serves as an indicator of T-lymphocyte response.