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Iron-chelating substances and inflammation.

G Döring1, T Pfestorf, K Botzenhart

  • 1Hygiene Institute, University of Tübingen, FRG.

Scandinavian Journal of Gastroenterology. Supplement
|January 1, 1988
PubMed
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Pseudomonas aeruginosa elastase (Ela) facilitates pyoverdin iron acquisition from transferrin by fragmenting it. Other proteases like alkaline protease (AP) and PMN elastase did not enable iron transfer.

Area of Science:

  • Microbiology
  • Biochemistry
  • Protease Function

Background:

  • * Iron acquisition is crucial for bacterial survival and virulence.
  • * Pyoverdin is a siderophore produced by Pseudomonas aeruginosa for iron uptake.
  • * Transferrin and lactoferrin are key iron-binding proteins in humans.

Purpose of the Study:

  • * To investigate the role of Pseudomonas aeruginosa alkaline protease (AP), elastase (Ela), and polymorphonuclear leukocyte elastase (PMN Ela) in pyoverdin-mediated iron acquisition.
  • * To determine the effect of these proteases on human transferrin and lactoferrin.

Main Methods:

  • * Incubation of iron-loaded transferrin and iron-free pyoverdin with AP, Ela, and PMN Ela at physiologic pH.
  • * Analysis of pyoverdin-iron complex formation.

Related Experiment Videos

  • * Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) to assess protein fragmentation.

    • Main Results:

    • * Ela facilitated the formation of a pyoverdin-iron(III) complex from transferrin.
    • * Ela fragmented transferrin into peptides < 14,000 daltons.
    • * AP and PMN Ela also fragmented transferrin but did not promote iron acquisition by pyoverdin.
    • * Lactoferrin fragmentation occurred with Ela and AP, but no iron acquisition by pyoverdin was observed.

    Conclusions:

    • * Pseudomonas aeruginosa elastase plays a significant role in enabling pyoverdin to acquire iron from transferrin through proteolysis.
    • * AP and PMN Ela are less effective in facilitating this iron acquisition process.
    • * Protease activity against lactoferrin does not lead to pyoverdin-mediated iron uptake.