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In vitro Cell Migration and Invasion Assays
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KMT2C modulates migration and invasion processes in osteosarcoma cell lines.

Caterina Chiappetta1, Raffaella Carletti1, Carlo Della Rocca1

  • 1UOC of Pathology, Department of Medical-Surgical Sciences and Bio-Technologies, Sapienza University of Rome, Latina, Italy.

Pathology, Research and Practice
|July 25, 2019
PubMed
Summary

KMT2C protein influences osteosarcoma cell movement and spread. Silencing KMT2C increased invasion in primary cells but enhanced migration in metastatic osteosarcoma cells, suggesting microenvironment-driven roles.

Keywords:
InvasionKMT2CMetastasisMigrationOsteosarcoma

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Area of Science:

  • Oncology
  • Molecular Biology
  • Epigenetics

Background:

  • Osteosarcoma is a primary bone cancer with significant metastatic potential.
  • Chromatin modifiers like KMT2C play roles in cancer progression, but their specific functions in osteosarcoma metastasis are not fully understood.

Purpose of the Study:

  • To investigate the role of KMT2C in osteosarcoma cell migration and invasion.
  • To determine if KMT2C function differs between primary and metastatic osteosarcoma cells.

Main Methods:

  • Osteosarcoma primary and metastatic cell lines were used.
  • KMT2C was silenced using siRNA.
  • Cell migration was assessed using wound healing and transwell assays.
  • Cell invasion was evaluated using Matrigel assays.

Main Results:

  • Primary osteosarcoma cells exhibited highest migration before KMT2C silencing and highest invasion after silencing.
  • Metastatic osteosarcoma cells showed highest migration after KMT2C silencing and highest invasion before silencing.
  • Differential effects of KMT2C modulation were observed between primary and metastatic cells.

Conclusions:

  • KMT2C plays distinct roles in the migration and invasion of primary versus metastatic osteosarcoma cells.
  • KMT2C-mediated enhancer changes may contribute to osteosarcoma metastasis, potentially influenced by the tumor microenvironment.