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4-Dimethylaminoazobenzene interferes with the binding of ribosomes to reticular membranes

R Gupta1, H M Dani

  • 1Department of Biochemistry, Panjab University, Chandigarh, India.

Toxicology Letters
|July 1, 1988
PubMed

Insights

The chemical 4-dimethylamino-azobenzene (4-DAB) impairs polyribosome binding to rough endoplasmic reticulum (RER) membranes. This suggests 4-DAB alters protein structures, hindering ribosome-RER interactions.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Background:

  • Rough endoplasmic reticulum (RER) is crucial for protein synthesis and folding.
  • Polyribosomes (polysomes) are complexes of mRNA and ribosomes involved in translation.
  • Understanding ribosome-membrane interactions is key to protein trafficking.

Purpose of the Study:

  • To investigate the effect of 4-dimethylamino-azobenzene (4-DAB) on the binding of polyribosomes to RER.
  • To determine if 4-DAB affects ribosome binding to stripped RER (DRER) fractions.

Main Methods:

  • Chemically degranulated RER was treated with 4-DAB.
  • The binding of fresh polyribosomes to reconstituted RER fractions was assessed.
  • Ribosomes were also treated with 4-DAB and their binding to DRER was evaluated.

Main Results:

  • 4-DAB treatment partially inhibited polyribosome binding to both lighter and heavier RER fractions.
  • 4-DAB treatment also inhibited ribosome binding to chemically stripped RER (DRER).

Conclusions:

  • 4-DAB interferes with the interaction between ribosomes and RER membranes.
  • The interaction of 4-DAB with RER and ribosomes may induce conformational changes in proteins, disrupting binding.

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