Related Experiment Video
Updated: Jan 21, 2026

Culturing Lymphocytes in Simulated Microgravity Using a Rotary Cell Culture System
Published on: August 25, 2022
Simulated microgravity induces nuclear translocation of Bax and BCL-2 in glial cultured C6 cells
Tommaso Bonfiglio1, Federico Biggi2, Anna Maria Bassi3
1Department of Internal Medicine, University of Genoa, Viale Benedetto XV 6, 16132 Genoa, Italy.
Abstract:
Alterations in the control of apoptotic processes were observed in cells during space flight or under simulated microgravity, the latter obtained with the 3D-Random Positioning Machine (3D-RPM). Usually the proteins Bax and Bcl-2, act as pro- or anti-apoptotic regulators. Here we investigated the effects of simulated microgravity obtained by the 3D-RPM on cell viability, localization and expression of Bax and Bcl-2 in cultures of glial cancerous cells. We observed for the first time a transient cytoplasmic/nuclear translocation of Bax and Bcl-2 triggered by changing gravity vector. Bax translocates into the nucleus after 1 h, is present simultaneously in the cytoplasm after 6 h and comes back to the cytoplasm after 24 h. Bcl-2 translocate into the nucleus only after 6 h and comes back to the cytoplasm after 24 h. Physiological meaning, on the regulation of apoptotic event and possible applicative outcomes of such finding are discussed.
Related Concept Videos
Glial Cells
Nervous Tissue: Glial Cells
The CNS glial cell includes the astrocytes, the oligodendrocytes, the microglia, and the ependymal cells.
Astrocytes are star-shaped glial...
Nuclear Fission
Non-nuclear Inheritance
Nuclear Stability
To hold positively charged protons together...
Nuclear Fusion
A helium nucleus has a mass that is 0.7% less than that of four hydrogen nuclei; this lost mass is converted into energy during the fusion. This reaction produces about...

