Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Quantum Numbers02:43

Quantum Numbers

49.4K
It is said that the energy of an electron in an atom is quantized; that is, it can be equal only to certain specific values and can jump from one energy level to another but not transition smoothly or stay between these levels.
49.4K
The Quantum-Mechanical Model of an Atom02:45

The Quantum-Mechanical Model of an Atom

56.7K
Shortly after de Broglie published his ideas that the electron in a hydrogen atom could be better thought of as being a circular standing wave instead of a particle moving in quantized circular orbits, Erwin Schrödinger extended de Broglie’s work by deriving what is now known as the Schrödinger equation. When Schrödinger applied his equation to hydrogen-like atoms, he was able to reproduce Bohr’s expression for the energy and, thus, the Rydberg formula governing hydrogen spectra.
56.7K
2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)01:19

2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)

1.4K
Heteronuclear single-quantum correlation spectroscopy (HSQC) is a 2D NMR technique that reveals one-bond correlations between hydrogen and a heteronucleus. The HSQC experiment is similar to the heteronuclear correlation experiment (HETCOR) but is more sensitive. In the HSQC spectrum, the proton chemical shift is plotted on the horizontal F2 axis, while the 13C chemical shift is plotted on the vertical F1 axis. The corresponding proton and 13C spectra are also shown. The HSQC contour plot does...
1.4K
Dot Product01:29

Dot Product

894
The dot product is an essential concept in mathematics and physics.
In engineering, the dot product of any two vectors is the product of the magnitudes of the vectors and the cosine of the angle between them. It is denoted by a dot symbol between the two vectors.
Consider a vehicle pulling an object along the ground using a rope. If the rope makes an angle with the horizontal axis, the work done can be calculated using the dot product of the force applied and the object's displacement.
The dot...
894
RNA Stability01:53

RNA Stability

35.6K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
35.6K
Dot Product: Problem Solving01:21

Dot Product: Problem Solving

685
The dot product is a powerful tool in problem-solving involving vectors, given that the dot product of two vectors is the product of their magnitudes and the cosine of the angle between them measured anti-clockwise. Solving problems involving the dot product requires understanding its properties and developing a step-by-step process to solve them. Here are the main steps to follow when solving any general problem involving the dot product:
Identify the problem: Start by reading the problem and...
685

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Efficient Decolorization of Molasses Wastewater by Thermophilic Carboxyl Ester Hydrolase: A New Immobilization Method.

Journal of food science·2026
Same author

SARS-CoV-2 enhances lysosomal exocytosis and deacidifies lysosomes to facilitate viral release.

mLife·2026
Same author

Silicon-Xanthenium Scaffold for pH- and Far-Red Light Dual-Responsive Photocage.

Journal of the American Chemical Society·2026
Same author

A Field-Deployable Microfluidic CNT-FET Platform for Direct Monitoring of Multiplexed Respiratory Viruses in Environmental Waters.

ACS sensors·2026
Same author

The tRNA landscape in cancer: from pathogenesis to therapeutic interventions.

Acta biochimica et biophysica Sinica·2026
Same author

Fusion with immunoglobulin Fc fragment enhances the immunogenicity of the African swine fever virus p30/p54 fusion protein.

Applied microbiology and biotechnology·2026

Related Experiment Video

Updated: Jan 21, 2026

Compact Quantum Dots for Single-molecule Imaging
17:14

Compact Quantum Dots for Single-molecule Imaging

Published on: October 9, 2012

18.7K

Quantum Dot Nanobeacons for Single RNA Labeling and Imaging.

Yingxin Ma1,2, Guobin Mao3, Weiren Huang2

  • 1State Key Laboratory of Virology, Wuhan Institute of Virology , Chinese Academy of Sciences , Wuhan , P. R. China.

Journal of the American Chemical Society
|July 25, 2019
PubMed
Summary

Researchers developed a novel quantum dot (QD) nanobeacon for sensitive detection and imaging of single RNA molecules in live cells, including HIV-1 RNA tracking.

More Related Videos

Real-time Imaging of Axonal Transport of Quantum Dot-labeled BDNF in Primary Neurons
10:53

Real-time Imaging of Axonal Transport of Quantum Dot-labeled BDNF in Primary Neurons

Published on: September 15, 2014

15.7K
Production and Targeting of Monovalent Quantum Dots
10:16

Production and Targeting of Monovalent Quantum Dots

Published on: October 23, 2014

26.0K

Related Experiment Videos

Last Updated: Jan 21, 2026

Compact Quantum Dots for Single-molecule Imaging
17:14

Compact Quantum Dots for Single-molecule Imaging

Published on: October 9, 2012

18.7K
Real-time Imaging of Axonal Transport of Quantum Dot-labeled BDNF in Primary Neurons
10:53

Real-time Imaging of Axonal Transport of Quantum Dot-labeled BDNF in Primary Neurons

Published on: September 15, 2014

15.7K
Production and Targeting of Monovalent Quantum Dots
10:16

Production and Targeting of Monovalent Quantum Dots

Published on: October 23, 2014

26.0K

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Nanotechnology

Background:

  • Detecting and imaging RNA in live cells presents significant challenges, especially for single RNA molecules.
  • Existing methodologies often lack the sensitivity and specificity required for real-time cellular analysis.

Purpose of the Study:

  • To develop a highly sensitive method for labeling and imaging individual RNA molecules within live cells.
  • To create a quantum dot (QD) nanobeacon platform for tracking low-abundance nucleic acids, including viral RNA.

Main Methods:

  • Constructed a quantum dot (QD) nanobeacon by conjugating black hole quencher (BHQ1) and phosphorothioate-modified DNA onto CdTe:Zn2+ QDs using a one-pot hydrothermal method.
  • Utilized nanobeacons with single DNA conjugation for labeling and detecting low-abundance nucleic acids.
  • Applied the method for single HIV-1 RNA detection and imaging in live HIV-1 integrated cells.

Main Results:

  • Successfully detected and imaged single HIV-1 RNAs in live cells.
  • Demonstrated that QD nanobeacon-labeled HIV-1 genomic RNAs can be encapsulated in progeny viral particles.
  • Showcased the potential for tracking the uncoating process of individual viruses.

Conclusions:

  • The developed QD nanobeacon platform offers high sensitivity for nucleic acid labeling and imaging.
  • This technology is particularly valuable for tracking individual RNA molecules in live cellular environments.
  • Provides a versatile platform for advancing live-cell RNA research and diagnostics.