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Updated: Jan 21, 2026

Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells
Published on: January 29, 2014
[Evaluation of the Optilite® analyser for determination of total complement activity and C3 and C4 fractions]
Benoit Nespola1, Hélène Comitogianni1, Isabelle Jahn1
1Laboratoire d'immunologie, Hôpitaux Universitaires de Strasbourg, France.
Abstract:
The complement system is composed of a set of plasma or membrane proteins. Complement protein deficiencies can be inherited or acquired, through the presence of autoantibodies or through consumption. We evaluated the analytical performance of the Optilite® analyser for the determination of the C3 and C4 levels and for the evaluation of the total complement activity. The intra- and inter-series CVs were evaluated and have showed satisfactory results, the concordances with analysers currently used in the laboratory (BNII® and BCT®, Siemens) are very good, as is the agreement between the serum and plasma samples. We also determined the reference values for the different parameters tested in view of a routine use of Optilite® analyser in the laboratory.
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