Related Experiment Video
Updated: Jan 21, 2026
09:36
RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
Published on: April 10, 2018
26.2K
Relationships of Non-coding RNA with diabetes and depression
Tian An1, Jing Zhang2, Yue Ma1
1Traditional Chinese Medicine School, Beijing University of Chinese Medicine, Beijing, 100029, China.
Scientific Reports
|July 26, 2019
Summary
Molecular differences in type 2 diabetes mellitus (T2DM) with depression (DD) were identified. Specific lncRNA, mRNA, and circRNA profiles in blood distinguish DD patients, revealing links to neuropsychiatric, immune, and inflammatory pathways.
Area of Science:
- Molecular biology
- Genomics
- Neuroscience
Background:
- Type 2 diabetes mellitus (T2DM) is a complex metabolic disorder.
- Comorbid depression (DD) in T2DM patients presents unique challenges.
- Understanding molecular distinctions between T2DM and DD is crucial for targeted therapies.
Purpose of the Study:
- To screen for differential expression of lncRNA, mRNA, and circRNA in the blood of T2DM patients with and without depression.
- To identify specific molecular markers associated with depression in T2DM.
- To investigate the functional roles of differentially expressed genes in DD.
Main Methods:
- Patient stratification using SDS, PHQ9, blood glucose, and HbA1c.
- lncRNA, mRNA, and circRNA expression profiling in peripheral blood.
- Bioinformatics analysis, including Gene Ontology (GO) and pathway analysis.
- RT-PCR validation of key lncRNAs and mRNAs.
Main Results:
- Identification of 28 differentially expressed lncRNAs, 107 circRNAs, and 89 mRNAs in the DD group.
- GO and pathway analysis revealed involvement in neuropsychiatric, immune, and inflammatory processes.
- RT-PCR confirmed the expression patterns of selected lncRNAs and mRNAs, validating sequencing data.
Conclusions:
- Distinct lncRNA, circRNA, and mRNA expression profiles characterize T2DM patients with depression.
- These molecular differences are linked to pathways involved in neuropsychiatric function, immunity, and inflammation.
- A complex lncRNA-mRNA regulatory network underlies DD pathogenesis in T2DM.
Related Concept Videos
02:39lncRNA - Long Non-coding RNAs
9.8K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
9.8K
01:05Long-term Depression
33.2K
Long-term depression, or LTD, is one of the ways by which synaptic plasticity—changes in the strength of chemical synapses—can occur in the brain. LTD is the process of synaptic weakening that occurs over time between pre and postsynaptic neuronal connections. The synaptic weakening of LTD works in opposition to synaptic strengthening by long-term potentiation (LTP) and together are the main mechanisms that underlie learning and memory.
33.2K
02:12Relationship Formation
45.2K
What do you think is the single most influential factor in determining with whom you become friends and whom you form romantic relationships? You might be surprised to learn that the answer is simple: the people with whom you have the most contact. This most important factor is proximity. You are more likely to be friends with people you have regular contact with. For example, there are decades of research that shows that you are more likely to become friends with people who live in your dorm,...
45.2K
03:12Freezing Point Depression and Boiling Point Elevation
39.6K
Boiling Point Elevation
The boiling point of a liquid is the temperature at which its vapor pressure is equal to ambient atmospheric pressure. Since the vapor pressure of a solution is lowered due to the presence of nonvolatile solutes, it stands to reason that the solution’s boiling point will subsequently be increased. Vapor pressure increases with temperature, and so a solution will require a higher temperature than will pure solvent to achieve any given vapor pressure, including one...
The boiling point of a liquid is the temperature at which its vapor pressure is equal to ambient atmospheric pressure. Since the vapor pressure of a solution is lowered due to the presence of nonvolatile solutes, it stands to reason that the solution’s boiling point will subsequently be increased. Vapor pressure increases with temperature, and so a solution will require a higher temperature than will pure solvent to achieve any given vapor pressure, including one...
39.6K
01:28Depressants
385
Depressant drugs, including alcohol and sedative-hypnotics, diminish central nervous system activity by enhancing the action of gamma-aminobutyric acid (GABA), a neurotransmitter that reduces brain activity and promotes relaxation. These substances can have various therapeutic uses but also pose significant risks, especially when misused or combined.
Alcohol is a common depressant that can induce a sense of relaxation and reduced inhibition at low doses. Contrary to its occasional...
Alcohol is a common depressant that can induce a sense of relaxation and reduced inhibition at low doses. Contrary to its occasional...
385
01:32RNA Splicing
60.4K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
60.4K