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Target Site Insensitivity Detection in Deltamethrin Resistant Culex pipiens Complex in Iran

Reza Zeidabadinezhad1, Hassan Vatandoost1,2, Mohammad Reza Abai1

  • 1Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.

Iranian Journal of Public Health
|July 26, 2019
PubMed
Abstract

Insights

Insecticide resistance in Culex pipiens mosquitoes, major vectors of West Nile virus, is driven by a specific mutation (L1014F) in the voltage-gated sodium channel gene. This finding aids in managing insecticide resistance and developing new vector control strategies.

Area of Science:

  • Medical Entomology
  • Molecular Entomology
  • Insecticide Resistance Studies

Background:

  • Culex pipiens mosquitoes are significant vectors for arboviruses like West Nile virus.
  • Knockdown resistance (kdr) mutations in the voltage-gated sodium channel (VGSC) gene confer resistance to pyrethroid insecticides.
  • The L1014F mutation is a known mechanism of insecticide resistance in various insect species.

Purpose of the Study:

  • To investigate the mechanisms of insecticide resistance in Culex pipiens populations.
  • To determine the presence and frequency of the L1014F kdr mutation in Cx. pipiens from Tehran.

Main Methods:

  • Mosquito larvae were collected from wastewater in Qarchak, Tehran.
  • Insecticide susceptibility tests were conducted using deltamethrin 0.05% following WHO guidelines.
  • Real-time PCR was employed to detect the specific L1014F point mutation in the VGSC gene.

Main Results:

  • High levels of resistance to deltamethrin were observed in the Cx. pipiens population.
  • Real-time PCR confirmed the presence of the resistant genotype (L1014F mutation) in all tested individuals.
  • The study identified a TTA to TTT nucleotide change at position L1014F in the VGSC gene.

Conclusions:

  • The L1014F mutation is a key mechanism of deltamethrin resistance in Cx. pipiens from Tehran.
  • This genetic information is crucial for effective insecticide resistance management strategies.
  • Findings support the need for novel approaches in arbovirus vector control programs.
Keywords:
Culex pipiensDeltamethrinKnockdown resistance

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