Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Clostridium difficile plasmid isolation as an epidemiologic tool.

C Clabots1, S Lee, D Gerding

  • 1Infectious Disease Section, Veterans Administration Medical Center, Minneapolis, Minnesota 55417.

European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology
|April 1, 1988
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Diphtheria in a congregate setting - facilitators and barriers to an effective Public Health response.

Irish medical journal·2025
Same author

Statistical data analysis of x-ray spectroscopy data enabled by neural network accelerated Bayesian inference.

The Review of scientific instruments·2024
Same author

Intra-islet glucagon signalling regulates beta-cell connectivity, first-phase insulin secretion and glucose homoeostasis.

Molecular metabolism·2024
Same author

Neratinib + fulvestrant + trastuzumab for HR-positive, HER2-negative, HER2-mutant metastatic breast cancer: outcomes and biomarker analysis from the SUMMIT trial.

Annals of oncology : official journal of the European Society for Medical Oncology·2023
Same author

7-Tesla MRI Evaluation of the Knee, 25 Years after Cartilage Repair Surgery: The Influence of Intralesional Osteophytes on Biochemical Quality of Cartilage.

Cartilage·2021
Same author

The role of cyclone activity in snow accumulation on Arctic sea ice.

Nature communications·2019
Same journal

Nocardia antimicrobial susceptibility testing by microdilution: evaluation of ready-to-use plate including tedizolid and meropenem.

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology·2026
Same journal

Consensus-based evaluation of RIDASCREEN<sup>®</sup> TB ELISA compared with two interferon-gamma release assays in a low-incidence setting.

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology·2026
Same journal

A review of bloodstream infections-pathogens, pathogenesis, diagnostic strategies, treatment methods-challenges and future aspects.

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology·2026
Same journal

Adaptive susceptibility shifts in Candidozyma auris following biocidal exposure: Exploratory effects on antifungal and biocidal susceptibility.

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology·2026
Same journal

Prevalence and risk factors of sexually transmitted infections in women in eleven hospitals in two low-income countries: a cross-sectional study.

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology·2026
Same journal

Tetracycline for Helicobacter pylori eradication: a narrative review from quadruple to dual therapy.

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology·2026
See all related articles

Plasmid profile typing helped trace a Clostridium difficile outbreak in a hospital. While other methods were more broadly applicable, plasmid analysis is useful for tracking specific Clostridium difficile infections, including reinfections and relapses.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Epidemiology

Background:

  • Clostridium difficile infection (CDI) is a significant cause of hospital-acquired diarrhea.
  • Effective outbreak investigation tools are crucial for controlling CDI spread within healthcare settings.

Purpose of the Study:

  • To evaluate the utility of plasmid profile typing for investigating a large hospital outbreak of Clostridium difficile.
  • To compare plasmid profiling with other typing methods for Clostridium difficile isolates.

Main Methods:

  • Plasmid DNA was extracted and analyzed from clinical isolates of Clostridium difficile obtained from patients during a hospital outbreak.
  • Isolates were also typed using bacteriophage/bacteriocin typing and polyacrylamide gel electrophoresis (PAGE)/bacterial agglutination typing for comparison.

Related Experiment Videos

Main Results:

  • Plasmids were successfully obtained from 37% of hospital outbreak isolates and 67% of non-hospital isolates.
  • Bacteriophage/bacteriocin and PAGE/bacterial agglutination typing demonstrated broader applicability across all isolates.

Conclusions:

  • Plasmid profile typing can be a valuable tool for tracing specific Clostridium difficile outbreaks, particularly for identifying reinfections and relapses caused by plasmid-bearing strains.
  • While not universally applicable, plasmid profiling complements other epidemiological tools for CDI outbreak investigations.