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Cloning and sequence analysis of human pituitary cDNA encoding the novel polypeptide 7B2
1Department of Animal Physiology, University of Nijmegen, The Netherlands.
FEBS Letters
|July 4, 1988
Summary
Researchers identified a human pituitary protein, 7B2, found in neurons and endocrine cells. This protein has structural features suggesting it may function as a GTP-binding protein.
Area of Science:
- Molecular Biology
- Endocrinology
- Neuroscience
Background:
- The polypeptide 7B2 is a protein found in secretory granules.
- Its selective presence in neurons and endocrine cells suggests a specialized function.
- The function of 7B2 has remained largely unknown.
Purpose of the Study:
- To isolate and characterize the human pituitary cDNA clone encoding polypeptide 7B2.
- To elucidate the structural features and potential function of the 7B2 protein.
Main Methods:
- Differential hybridization technique was employed for cDNA isolation.
- Amino acid sequence analysis was performed on the isolated 7B2 polypeptide.
- Bioinformatic analysis identified conserved structural domains.
Main Results:
- A human pituitary cDNA clone encoding the complete structure of polypeptide 7B2 was isolated.
- Human 7B2 comprises 185 amino acids with a cleaved signal peptide.
- Structural analysis revealed a serine phosphorylation site, potential proteolytic cleavage sites, and three GTP-binding domains.
Conclusions:
- The 7B2 protein is highly conserved and present in specific cell types.
- Structural homology to GTP-binding domains suggests 7B2 may function as a GTP-binding protein.
- Further research is warranted to determine the precise function of 7B2 in cellular processes.