Related Experiment Video

Updated: Jan 21, 2026

Isolation, Characterization, and Total DNA Extraction to Identify Endophytic Fungi in Mycoheterotrophic Plants
06:53

Isolation, Characterization, and Total DNA Extraction to Identify Endophytic Fungi in Mycoheterotrophic Plants

Published on: May 5, 2023

4.0K

[Inhibitory effect of endophytic fungi from Dysosma versipellis on HIV-1 IN-LEDGF/p75 interaction]

Ya-Qin Zhou1, Da-Wei Zhang2, Li-Ying Yu3

  • 1Guangxi University of Chinese Medicine Nanning 530200,China Guangxi Botanical Garden of Medicinal Plant Nanning 530023,China.

Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica
|July 26, 2019
PubMed

Insights

Endophytic fungi from Dysosma versipellis were screened for activity against HIV-1 integrase and LEDGF/p75 interaction. One strain showed strong inhibition, offering potential for new AIDS drug development.

Area of Science:

  • Mycology
  • Virology
  • Drug Discovery

Background:

  • The interaction between HIV-1 integrase (IN) and LEDGF/p75 is a critical target for antiviral therapies.
  • Endophytic fungi represent a rich source of novel bioactive compounds.

Purpose of the Study:

  • To identify endophytic fungi from Dysosma versipellis with inhibitory activity against the HIV-1 IN-LEDGF/p75 interaction.
  • To characterize and identify potent fungal strains for potential anti-HIV drug development.

Main Methods:

  • Screening of 53 endophytic fungal strains from D. versipellis using homogeneous time-resolved fluorescence (HTRF) assay.
  • Identification of active strains using ITS-rDNA, LSU, and RPB2 sequence analysis.

Main Results:

  • Eight endophytic fungal strains exhibited inhibitory activity against the HIV-1 IN-LEDGF/p75 interaction.
  • Strain 106 demonstrated potent inhibition with an IC50 value of 5.23 mg·L-1.
  • Strain 106 was identified as a potential novel species within the Magnaporthaceae family.

Conclusions:

  • Endophytic fungi of D. versipellis harbor compounds that can inhibit the crucial HIV-1 IN-LEDGF/p75 interaction.
  • The identified potent strain (106) represents a promising resource for developing new anti-acquired immunodeficiency syndrome (AIDS) drugs.
Keywords:
Dysosma versipellisHIV-1LEDGF/p75endophytic fungiintegraseprotein-protein interaction

More Related Videos

Establishing Fungal Entomopathogens as Endophytes: Towards Endophytic Biological Control15:14

Establishing Fungal Entomopathogens as Endophytes: Towards Endophytic Biological Control

Published on: April 11, 2013

35.4K
Genotypic Inference of HIV-1 Tropism Using Population-based Sequencing of V311:10

Genotypic Inference of HIV-1 Tropism Using Population-based Sequencing of V3

Published on: December 27, 2010

12.7K

Related Concept Videos

The Roles of Bacteria and Fungi in Plant Nutrition02:11

The Roles of Bacteria and Fungi in Plant Nutrition

Plants have the impressive ability to create their own food through photosynthesis. However, plants often require assistance from organisms in the soil to acquire the nutrients they need to function correctly. Both bacteria and fungi have evolved symbiotic relationships with plants that help the species to thrive in a wide variety of environments.
47.1K
Overview of Fungi01:29

Overview of Fungi

Fungi are a diverse group of eukaryotes more closely related to animals than other eukaryotes. Fungal cell walls comprise chitin, a polysaccharide that provides structural strength, and glucans, which contribute to flexibility and integrity. Other polysaccharides, such as mannans and galactosans, may supplement or replace chitin in some fungi. These adaptations, along with their preference for acidic environments and tolerance for high osmotic pressure, enable fungi to thrive in various...
1.6K
Excitatory and Inhibitory Effects of Neurotransmitters01:29

Excitatory and Inhibitory Effects of Neurotransmitters

When an action potential reaches the presynaptic axon terminal, it releases neurotransmitters from the neuron into the synaptic cleft at a chemical synapse. The released neurotransmitter can be excitatory or inhibitory. The critical criteria commonly used to determine whether a molecule is a neurotransmitter at a chemical synapse are the molecule's presence in the presynaptic neuron. Second, its release is in response to strong presynaptic depolarization. And lastly, the presence of...
12.7K
Predator-Prey Interactions02:39

Predator-Prey Interactions

Predators consume prey for energy. Predators that acquire prey and prey that avoid predation both increase their chances of survival and reproduction (i.e., fitness). Routine predator-prey interactions elicit mutual adaptations that improve predator offenses, such as claws, teeth, and speed, as well as prey defenses, including crypsis, aposematism, and mimicry. Thus, predator-prey interactions resemble an evolutionary arms race.
21.1K
SN1 Reaction: Stereochemistry02:15

SN1 Reaction: Stereochemistry

This lesson provides an in-depth discussion of the stereochemical outcomes in an SN1 reaction.
In the first step of an SN1 reaction, the bond between the electrophilic carbon and the leaving group ionizes to generate the carbocation intermediate. The second step of the mechanism is the nucleophilic attack.
In the formed carbocation, the positively charged carbon is sp2 hybridized with a trigonal planar geometry. As all the three substituents lie on the same plane, a plane of symmetry for the...
10.2K
SN1 Reaction: Kinetics02:05

SN1 Reaction: Kinetics

In an SN2 reaction, the reaction rate depends on both the type of nucleophile and the substrate. A hindered tertiary alkyl halide is practically inert to the SN2 mechanism despite using a strong nucleophile.
However, Sir Christopher Ingold and Edward D. Hughes, who studied the kinetics of various nucleophilic substitution reactions, noticed that a tertiary alkyl halide does undergo a nucleophilic substitution reaction in the presence of a weak nucleophile. While studying the substitution...
9.5K