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Updated: Jan 21, 2026

Microtiter Dish Biofilm Formation Assay
Published on: January 30, 2011
Stick or leave - Pushing methanogens to biofilm formation for ex situ biomethanation
Mads Borgbjerg Jensen1, Dietmar Strübing2, Nadieh de Jonge3
1Department of Engineering, Aarhus University, Hangøvej 2, DK-8200 Aarhus N., Denmark.
Abstract:
Biomethanation exploits the ability of methanogenic archaea to convert CO2 and renewable H2 from electrolysis to biomethane. Biofilm reactors are promising for biomethanation scale-up due to high CH4 productivity and low energy input for H2 gas-liquid mass transfer. Effects of operational conditions on biofilm dynamics remain largely uncharacterized but may increase reactor potentials further. This study investigated the effect of hydraulic retention time (HRT) on methanogenic biofilm activity and composition. Commercial carriers floating in liquid were exposed to H2/CO2 for 87 days with the liquid phase being subject to either 18 hours, 10 days, or 20 days HRT. Methanogenic biofilms were dominated by hydrogenotrophic methanogens, but biofilm CH4 productivity was enhanced at 18 hours HRT due to wash-out of competing planktonic species, which otherwise hampered proliferation of biofilm biomass at long HRT. It is suggested that high-rate biofilm reactors can increase methanogenic biofilm activity by minimizing the liquid's H2 exposure.
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