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Updated: Jan 21, 2026
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Directed Dopaminergic Neuron Differentiation from Human Pluripotent Stem Cells
Published on: September 15, 2014
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HuR interacts with lincBRN1a and lincBRN1b during neuronal stem cells differentiation
Stephana Carelli1,2, Toniella Giallongo1, Federica Rey1
1Laboratory of Pharmacology, Department of Health Sciences, University of Milan , Milan , Italy.
RNA Biology
|July 27, 2019
Summary
Long non-coding RNAs (lncRNAs) and HuR protein are key regulators of neural stem cell (NSC) differentiation. Their synergistic action influences neuronal fate during brain development.
Area of Science:
- Neuroscience
- Molecular Biology
- Stem Cell Biology
Background:
- Long non-coding RNAs (lncRNAs) are critical regulators of cellular processes.
- The role of lncRNAs in adult brain and neural stem cells (NSCs) differentiation is not fully understood.
Purpose of the Study:
- To investigate the role of specific lncRNAs and their interaction with ELAVL1/HuR during NSC differentiation.
- To characterize the function of lincBRN1a and lincBRN1b in neuronal differentiation.
Main Methods:
- Expression analysis of 10 lncRNAs during murine NSC differentiation.
- Functional characterization of lincBRN1a and lincBRN1b inhibition.
- Identification and analysis of human lncRNA homologs during human iPSC differentiation.
Main Results:
- Ten lncRNAs were found to be expressed during murine NSC differentiation and interact with HuR.
- Inhibition of lincBRN1a and lincBRN1b promoted NSC differentiation, reducing stemness and increasing neuronal markers.
- HuR was shown to regulate the half-life of these lncRNAs, suggesting a synergistic role.
- Six human lncRNA homologs were identified and found to be deregulated during human iPSC differentiation into neurons.
Conclusions:
- lncRNAs and HuR play a crucial synergistic role in regulating NSC differentiation and neuronal fate.
- These findings highlight the importance of lncRNA-HuR interactions in neurogenesis.
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